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Identification of abnormally high expression of POGZ as a new biomarker associated with a poor prognosis in osteosarcoma
European Journal of Histochemistry ( IF 2 ) Pub Date : 2021-09-03 , DOI: 10.4081/ejh.2021.3264
Sikuan Zheng 1 , Yue Liu 2 , Haohe Sun 3 , Jingyu Jia 4 , Tianlong Wu 1 , Rui Ding 4 , Xigao Cheng 4
Affiliation  

Osteosarcoma (OS) is the most prevalent malignant bone tumor in children and young adults. There is an urgent need for a novel biomarker related to the prognosis of OS. We performed a meta-analysis incorporating six independent datasets and performed a survival analysis with one independent dataset GSE21257 in the GEO database for gene screening. The results revealed that one potential biomarker related to OS survival, POGZ was the most significantly upregulated gene. We also verified that the POGZ was overexpressed in clinical samples. The survival analysis revealed that POGZ is associated with a poor prognosis in OS. Moreover, flow cytometry analysis of isolated OS cells demonstrated that OS cells were arrested in the G1 phase after POGZ knockdown. The RNA-seq results indicated that POGZ was co-expressed with CCNE1 and CCNB1. Pathway analysis showed that genes associated with high expression levels of POGZ were related to the cell cycle pathway. A cell model was constructed to detect the effects of POGZ. After POGZ knockdown, OS cell proliferation, invasion and migration were all decreased. Therefore, POGZ is an important gene for evaluating the prognosis of OS patients and is a potential therapeutic target.



中文翻译:

将 POGZ 的异常高表达鉴定为与骨肉瘤预后不良相关的新生物标志物

骨肉瘤(OS)是儿童和年轻人中最常见的恶性骨肿瘤。迫切需要一种与 OS 预后相关的新型生物标志物。我们进行了包含六个独立数据集的荟萃分析,并使用 GEO 数据库中的一个独立数据集 GSE21257 进行了生存分析,用于基因筛选。结果显示,与 OS 存活相关的一种潜在生物标志物POGZ是最显着上调的基因。我们还验证了POGZ在临床样本中过度表达。生存分析显示,POGZ与 OS 预后不良有关。此外,分离的 OS 细胞的流式细胞术分析表明,在POGZ后 OS 细胞在 G1 期停滞。击倒。RNA-seq 结果表明POGZ与 CCNE1 和 CCNB1 共表达。通路分析表明,与POGZ高表达水平相关的基因与细胞周期通路有关。构建细胞模型以检测POGZ的影响。POGZ敲低后,OS细胞增殖、侵袭和迁移均下降。因此,POGZ是评价OS患者预后的重要基因,是潜在的治疗靶点。

更新日期:2021-09-03
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