A series of Cu(II) complexes were synthesized by using N-hydroxy-N,N′-diarylformamidine ligands: N-hydroxy-N,N′-(phenyl)formamidine (L1), N-hydroxy-N′-(4-methylphenyl)formamidine (L2), N-hydroxy-N,N′-(2,6-dimethylphenyl)formamidine (L3), N-hydroxy-N,N′-(2,6-diisopropylphenyl)formamidine (L4). Reaction of ligands L1–L4 with hydrated copper acetate furnished mononuclear Cu(II) complexes 1–4 with general formula [Cu-(L)₂]. The molecular structures of complexes 3 and 4, as determined by single crystal X-ray diffraction, showed both to have square planar geometry with a near C₂ symmetry. The antimicrobial potency of all four complexes was evaluated against three gram-(–) bacteria (S. typhimurium, P. aeruginosa, and E. coli) and two gram-(+) bacteria (Methicillin-resistant S. aureus (MRSA) and S. aureus), with ciprofloxacin as the reference drug. All tested complexes were inactive against gram-(+) bacteria strains except for complex 1, which displayed excellent activity when compared to the reference. Molecular docking studies showed that hydrogen bonding, pi-sigma and van der Waals interactions are prominent complex-protein connections, with complex 2 displaying good binding affinities with the studied biological targets.

使用N-羟基-N、N'-二芳基甲脒配体合成了一系列Cu(II)配合物：N-羟基-N、N' -(苯基)甲脒( L1 )、N-羟基-N' -(4 -甲基苯基）甲脒（L2）、N-羟基-N、N' -（2,6-二甲基苯基）甲脒（L3）、N-羟基-N,N' -（2,6-二异丙基苯基）甲脒（L4）。配体L1 – L4的反应与水合乙酸铜配制成单核 Cu(II) 配合物1-4，通式为 [Cu-( _L ) 2 ]。配合物3和4的分子结构，通过单晶 X 射线衍射测定，均显示出具有接近C ₂对称性的方形平面几何形状。评估了所有四种复合物对三种革兰氏 (-) 细菌（鼠伤寒沙门氏菌、铜绿假单胞菌和大肠杆菌）和两种革兰氏 (+) 细菌（耐甲氧西林金黄色葡萄球菌 (MRSA ) 和金黄色葡萄球菌)，以环丙沙星为参照药。除复合物1外，所有测试的复合物都对革兰氏 (+) 细菌菌株无活性，与参考相比，复合物 1 显示出优异的活性。分子对接研究表明，氢键、π-σ 和范德华相互作用是突出的复合物-蛋白质连接，复合物2与所研究的生物靶标具有良好的结合亲和力。