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Flavanols and Flavanes from Crinum asiaticum and Their Effects on LPS Signaling Pathway Through the Inhibition of NF-κB Activation
Planta Medica ( IF 2.7 ) Pub Date : 2021-09-02 , DOI: 10.1055/a-1585-5877
Kiep Minh Do 1 , Min-Kyoung Shin 1 , Takeshi Kodama 1 , Nwet Nwet Win 1 , Prema Prema 1, 2 , Hien Minh Nguyen 3 , Yoshihiro Hayakawa 1 , Hiroyuki Morita 1
Affiliation  

Three new flavanols, (2R,3S)-7-methoxy-flavan-3-ol (1), (2R,3S)-7-hydroxy-flavan-3-ol (2), and (2R,3S)-2′-hydroxy-7-methoxy-flavan-3-ol (3), together with two known flavans (4 and 5), were isolated from the chloroform extract of Crinum asiaticum. Their structures were elucidated by various spectroscopic methods, including 1D and 2D NMR, HR-ESI-MS, and CD data. The isolated compounds 1 and 3−5 showed inhibitory activity toward LPS-induced nitric oxide (NO) production. Further investigation of the NF-κB pathway mechanisms indicated that 1 and 3−5 inhibited the LPS-induced IL-6 production and p65 subunit phosphorylation of NF-κB in RAW264.7 cells, with an effective dose of 10 µM.

中文翻译:

Crinum asiaticum 中的黄烷醇和黄烷及其通过抑制 NF-κB 活化对 LPS 信号通路的影响

三种新黄烷醇,(2R,3S)-7-methoxy-flavan-3-ol (1)、(2R,3S)-7-hydroxy-flavan-3-ol (2) 和 (2R,3S)-2 '-hydroxy-7-methoxy-flavan-3-ol (3) 与两种已知的黄烷(4 和 5)一起从 Crinum asiaticum 的氯仿提取物中分离出来。它们的结构通过各种光谱方法进行了阐明,包括 1D 和 2D NMR、HR-ESI-MS 和 CD 数据。分离的化合物 1 和 3-5 对 LPS 诱导的一氧化氮 (NO) 产生具有抑制活性。对 NF-κB 通路机制的进一步研究表明,1 和 3-5 在 RAW264.7 细胞中抑制 LPS 诱导的 IL-6 产生和 NF-κB 的 p65 亚基磷酸化,有效剂量为 10 µM。
更新日期:2021-09-03
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