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Immunoassay based on Au-Ag bimetallic nanoclusters for colorimetric/fluorescent double biosensing of dicofol
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2021-09-03 , DOI: 10.1016/j.bios.2021.113611
Yi Pan 1 , Xinlin Wei 1 , Xiaodong Guo 1 , Hui Wang 2 , Haiyun Song 2 , Canping Pan 3 , Naifeng Xu 4
Affiliation  

The high toxicity of dicofol (DICO) to nontarget organisms has resulted in the contamination of food materials and caused a threat to human health. Developing a rapid and sensitive detection method of DICO in food samples is essential and still pursued. Fluorescent nanomaterials have been widely applied in biosensors to improve the sensitivity of detection. Herein, glutathione-capped Au-Ag bimetallic nanoclusters (Au-Ag NCs) exhibited the outstanding fluorescence characteristic with the average fluorescence lifetime of 1971.08 ns and photoluminescence quantum yield of 9.84% when the molar ratio of Au to Ag was 5:1. Polyethyleneimine modified gold nanoparticles (PEI-Au NPs) with the positive charge were prepared to generate a strong colorimetric signal. A dual-model colorimetric/fluorescent immune probe based on the Au-Ag NCs and PEI-Au NPs was successfully constructed by electrostatic force, and could be applied in both ic-ELISA and LFIA methods for rapid and ultrasensitive detection of DICO. In the ic-ELISA method, the introduction of fluorescence signal significantly increased the sensitivity of detection with the limit of detection (LOD) of 0.62 ng/mL and exhibited an excellent linear relationship within the range of 1.36 ng/mL-19.92 ng/mL. In the LFIA method, the fluorescence signal of Au-Ag NCs was accumulated on the test line and control line for the fluorescence model detection with a quantitative LOD at the level of 1.59 ng/mL. Such a dual-model colorimetric/fluorescent immunoassay serves as a promising candidate to develop new approaches in field detection.



中文翻译:

基于 Au-Ag 双金属纳米团簇的免疫测定法用于三氯杀螨醇的比色/荧光双重生物传感

三氯杀螨醇 (DICO) 对非靶标生物的高毒性已导致食品材料受到污染,并对人类健康造成威胁。开发一种快速、灵敏的食品样品中 DICO 检测方法是必不可少的,并且仍在继续。荧光纳米材料已广泛应用于生物传感器以提高检测灵敏度。在此,谷胱甘肽封端的 Au-Ag 双金属纳米团簇(Au-Ag NCs)表现出优异的荧光特性,当 Au 与 Ag 的摩尔比为 5:1 时,平均荧光寿命为 1971.08 ns,光致发光量子产率为 9.84%。制备带有正电荷的聚乙烯亚胺改性金纳米粒子 (PEI-Au NPs) 以产生强比色信号。通过静电力成功构建了基于 Au-Ag NCs 和 PEI-Au NPs 的双模型比色/荧光免疫探针,可用于 ic-ELISA 和 LFIA 方法对 DICO 进行快速和超灵敏检测。在ic-ELISA方法中,荧光信号的引入显着提高了检测灵敏度,检测限(LOD)为0.62 ng/mL,并在1.36 ng/mL-19.92 ng/mL范围内表现出良好的线性关系. 在 LFIA 方法中,Au-Ag NCs 的荧光信号在测试线和控制线上累积,用于荧光模型检测,定量 LOD 为 1.59 ng/mL。这种双模型比色/荧光免疫测定法是开发现场检测新方法的有希望的候选者。

更新日期:2021-09-03
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