Expert Review of Proteomics ( IF 3.4 ) Pub Date : 2021-09-11 , DOI: 10.1080/14789450.2021.1976150 Natalia Zakharova 1 , Anna Kozyr 1 , Anna M Ryabokon 1, 2 , Maria Indeykina 1, 3 , Polina Strelnikova 1 , Anna Bugrova 1 , Eugene N Nikolaev 4 , Alexey S Kononikhin 1, 4
ABSTRACT
Introduction
Lung cancer remains the most prevalent cause of cancer mortality worldwide mainly due to insufficient availability of early screening methods for wide-scale application. Exhaled breath condensate (EBC) is currently considered as one of the promising targets for early screening and is particularly attractive due to its absolutely noninvasive collection and possibility for long-term frozen storage. EBC proteome analysis can provide valuable information about the (patho)physiological changes in the respiratory system and may help to identify in time a high risk of lung cancer. Mass spectrometry (MS) profiling of EBC proteome seems to have no alternative in obtaining the most extensive data and characteristic marker panels for screening.
Areas covered
This special report summarizes the data of several proteomic studies of EBC in normal and lung cancer (from 2012 to 2021, PubMed), focuses on the possible reasons for the significant discrepancy in the results, and discusses some aspects for special attention in further studies.
Expert opinion
The significant discrepancy in the results of various studies primarily highlights the need to create standardized protocols for the collection and preparation of EBC for proteomic analysis. The application of quantitative and targeted LC-MS/MS based approaches seems to be the most promising in further EBC proteomic studies.
中文翻译:
基于质谱的呼出气冷凝物蛋白质组分析用于肺癌生物标志物搜索
摘要
介绍
肺癌仍然是全球癌症死亡的最普遍原因,主要是由于早期筛查方法无法广泛应用。呼出气冷凝液(EBC)目前被认为是早期筛查的有希望的目标之一,并且由于其绝对无创收集和长期冷冻储存的可能性而特别有吸引力。EBC 蛋白质组分析可以提供有关呼吸系统(病理)生理变化的宝贵信息,并可能有助于及时识别肺癌的高风险。EBC 蛋白质组的质谱 (MS) 分析似乎无法获得最广泛的数据和用于筛选的特征标记组。
涵盖的领域
本专题报告总结了 EBC 在正常和肺癌中的几项蛋白质组学研究(从 2012 年到 2021 年,PubMed)的数据,重点分析了结果存在显着差异的可能原因,并讨论了进一步研究中需要特别注意的一些方面。
专家意见
各种研究结果的显着差异主要强调了为收集和准备 EBC 以进行蛋白质组学分析创建标准化协议的必要性。在进一步的 EBC 蛋白质组学研究中,基于定量和靶向 LC-MS/MS 的方法的应用似乎是最有希望的。