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Isolation and characterization of novel phage (Podoviridae ɸParuNE1) and its efficacy against multi-drug-resistant Pseudomonas aeruginosa planktonic cells and biofilm
Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2021-09-03 , DOI: 10.1186/s43088-021-00137-4
Nkechi V. Enwuru 1 , Jason J. Gill 2 , Katri P. Anttonen 3 , Jeffrey D. Cirillo 3 , Christian A. Enwuru 4 , Ry. Young 5 , Akinloye O. Coker 6
Affiliation  

Bacterial pathogen (Pseudomonas aeruginosa) could form biofilm that conveys multi-drug resistance. Bacteriophage as an alternative to antibacterial resistance is useful against biofilm complications. This study evaluated antibacterial and biofilm removal activities of lytic phage, specific against multi-drug-resistant clinical P. aeruginosa. The phage showed a wide range of pH (5–10) and heat (7–44 °C) stability. Electron microscopy showed ɸPauNE1 phage head (60 nm in diameter) and non-contractile tail (12 nm in length by 8 nm in width); hence, the family Podoviridae and the order Caudovirales. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed structured protein of 55 kDa and double-stranded DNA of 45 kb. The phage was species specific and had broad host range activity. It inhibited bacterial growth at multiplicity of infection (MOI) 1–0.000001 pfu/ml. Inhibition was maximal at both low (1 × 105) and high (1 × 109) bacterial CFU/ml. Biofilm removal test showed that the phage removed more than 60% cell biomass within CFU/ml of 1.5 × 108, 6.0 × 108 and l.0 × 109. Phage (ɸPauNE1) was unique and had broad host range activity. The phage exhibited strong bacteriolytic activity against biofilm forming multi-drug-resistant strains. It had no lytic effect on the heterogeneous strains and so a promising bioagent.

中文翻译:

新型噬菌体(Podoviridae ɸParuNE1)的分离和表征及其对多重耐药铜绿假单胞菌浮游细胞和生物膜的功效

细菌病原体(铜绿假单胞菌)可以形成生物膜,传递多药耐药性。噬菌体作为抗菌抗性的替代品可用于对抗生物膜并发症。本研究评估了裂解噬菌体的抗菌和生物膜去除活性,特异性针对多重耐药的临床铜绿假单胞菌。噬菌体显示出广泛的 pH (5-10) 和热 (7-44 °C) 稳定性。电子显微镜显示 ɸPauNE1 噬菌体头部(直径 60 nm)和非收缩尾部(长 12 nm,宽 8 nm);因此,家庭Podoviridae和Caudovirales目。十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 显示 55 kDa 的结构蛋白和 45 kb 的双链 DNA。该噬菌体具有物种特异性并具有广泛的宿主范围活性。它在感染复数 (MOI) 1–0.000001 pfu/ml 时抑制细菌生长。在低 (1 × 105) 和高 (1 × 109) 细菌 CFU/ml 时抑制作用最大。生物膜去除试验表明,噬菌体在 1.5 × 108、6.0 × 108 和 l.0 × 109 的 CFU/ml 内去除了超过 60% 的细胞生物量。噬菌体 (ɸPauNE1) 是独特的,具有广泛的宿主范围活性。噬菌体对形成多重耐药菌株的生物膜表现出很强的溶菌活性。它对异源菌株没有裂解作用,因此是一种很有前途的生物制剂。噬菌体对形成多重耐药菌株的生物膜表现出很强的溶菌活性。它对异源菌株没有裂解作用,因此是一种很有前途的生物制剂。噬菌体对形成多重耐药菌株的生物膜表现出很强的溶菌活性。它对异源菌株没有裂解作用,因此是一种很有前途的生物制剂。
更新日期:2021-09-03
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