Bacterial infection remains a great challenge in wound healing, especially in chronic wounds. Multidrug-resistant organisms are increasing in acute and chronic wound infections, which compromise the chance of therapeutics. Resistance to conventional antibiotics has created an urge to study new approach/system that can effectively control wound infection and enhance healing. Wound cover/dressing must exhibit biocompatibility and effectiveness in reducing bioburden at the wound site. Collagen, a natural biopolymer, possesses advantages over synthetic and other natural materials due to its unique biological properties. It can act as an excellent wound dressing and controlled drug delivery system. Currently, antiseptic agents such as silver, iodine, and polyhexamethylene biguanide (PHMB)-incorporated scaffolds have become widely accepted in chronic wound healing. In this study, PHMB-incorporated collagen scaffold has been prepared and characterized using Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD), and differential scanning calorimetry (DSC), which showed retention of collagen nativity and integration of PHMB. The scanning electron microscopy (SEM) analysis revealed the porous structures of scaffolds. The cytotoxicity analysis showed PHMB is nontoxic at the concentration of 0.01% (wt/wt). The agar diffusion test and bacterial adhesion study demonstrated the effectiveness of PHMB-incorporated collagen scaffold against both gram positive and negative strains. This study concludes that PHMB-incorporated collagen scaffold could have the potential for infected wound healing.

中文翻译：

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用聚六亚甲基双胍制备胶原蛋白：感染伤口的潜在支架

细菌感染仍然是伤口愈合的一大挑战，特别是在慢性伤口中。在急性和慢性伤口感染中，耐多药生物体正在增加，这损害了治疗的机会。对传统抗生素的耐药性催生了研究能够有效控制伤口感染和促进愈合的新方法/系统的冲动。伤口覆盖物/敷料必须表现出生物相容性和减少伤口部位生物负载的有效性。胶原蛋白是一种天然生物聚合物，由于其独特的生物学特性，比合成材料和其他天然材料具有优势。它可以作为一种极好的伤口敷料和受控的药物输送系统。目前，防腐剂如银、碘、和聚六亚甲基双胍 (PHMB) 结合的支架在慢性伤口愈合中已被广泛接受。在本研究中，制备了掺入 PHMB 的胶原蛋白支架，并使用傅里叶变换红外光谱 (FTIR)、圆二色性 (CD) 和差示扫描量热法 (DSC) 对其进行了表征，结果表明胶原蛋白的天然保留和 PHMB 的整合。扫描电子显微镜 (SEM) 分析揭示了支架的多孔结构。细胞毒性分析表明 PHMB 在 0.01% (wt/wt) 的浓度下是无毒的。琼脂扩散试验和细菌粘附研究证明了掺入 PHMB 的胶原蛋白支架对革兰氏阳性和阴性菌株的有效性。