BACKGROUND Hirschsprung's disease (HSCR) is a common defect in newborns, and studies have revealed that long non-coding RNA (lncRNA) is involved in the progression of HSCR. This research study aims to investigate the mechanism of downregulated RNA in cancer (DRAIC) on cell proliferation and migration in HSCR. METHODS Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the expression of DRAIC in HSCR bowel stenosis tissues and normal colon tissues. Cell-counting kit-8 (CCK-8) and Transwell assays were employed to explore whether cellular functions change after overexpression or knockdown of the DRAIC in SH-SY5Y cells and human 293T cells. Protein expression levels were determined by Western blot analysis. RNA pull-down and dual-luciferase reporter assays were used to confirm the competitive relationship of DRAIC and integrin subunit alpha 6 (ITGA6) through their association with miR-34a-5p. RESULTS The lncRNA DRAIC was significantly increased in colon tissue from HSCR patients. The overexpression of DRAIC inhibited SH-SY5Y cell and human 293T cell proliferation and migration. Knockdown of DRAIC, however, promoted cell proliferation and migration. The RNA pull-down and dual-luciferase reporter assays have proven the competitive relationship between DRAIC and ITGA6 through their association with miR-34a-5p. Further rescue experiments have confirmed that DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in HSCR. CONCLUSION DRAIC promoted cell proliferation and migration by regulating the miR-34a-5p/ITGA6 signal axis in HSCR.

中文翻译：

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LncRNA DRAIC 通过影响先天性巨结肠的 miR-34a-5p/ITGA6 信号轴来调节细胞增殖和迁移。

背景先天性巨结肠（HSCR）是新生儿常见的缺陷，研究表明长非编码RNA（lncRNA）参与HSCR的进展。本研究旨在探讨癌症中下调的RNA（DRAIC）对HSCR细胞增殖和迁移的作用。方法采用定量逆转录-聚合酶链反应(qRT-PCR)检测HSCR肠狭窄组织和正常结肠组织中DRAIC的表达。采用细胞计数 kit-8 (CCK-8) 和 Transwell 测定来探索在 SH-SY5Y 细胞和人 293T 细胞中过表达或敲低 DRAIC 后细胞功能是否发生变化。通过蛋白质印迹分析确定蛋白质表达水平。RNA pull-down 和双荧光素酶报告基因分析用于通过与 miR-34a-5p 的关联来确认 DRAIC 和整合素亚基 alpha 6 (ITGA6) 的竞争关系。结果 HSCR 患者结肠组织中的 lncRNA DRAIC 显着增加。DRAIC的过表达抑制了SH-SY5Y细胞和人293T细胞的增殖和迁移。然而，DRAIC 的敲低促进了细胞增殖和迁移。RNA pull-down 和双荧光素酶报告基因分析通过与 miR-34a-5p 的关联证明了 DRAIC 和 ITGA6 之间的竞争关系。进一步的拯救实验证实，DRAIC 通过影响 HSCR 中的 miR-34a-5p/ITGA6 信号轴来调节细胞增殖和迁移。