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Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes
The Journal of Infectious Diseases ( IF 6.4 ) Pub Date : 2021-01-26 , DOI: 10.1093/infdis/jiab056 Mahamoudou Ouattara 1 , Mamadou Tamboura 2 , Dinanibè Kambiré 2 , Kim Anh Lê 3 , Thanh Van Phan 4 , Srinivasan Velusamy 1 , Hien Anh Nguyen 3 , Dai Vo Thi Trang 4 , Fernanda C Lessa 1 , Makiko Iijima 5 , Dac Trung Nguyen 3 , Stephanie B Schwartz 1 , Lesley McGee 1 , Rasmata Ouédraogo Traoré 2 , Bernard Beall 1
The Journal of Infectious Diseases ( IF 6.4 ) Pub Date : 2021-01-26 , DOI: 10.1093/infdis/jiab056 Mahamoudou Ouattara 1 , Mamadou Tamboura 2 , Dinanibè Kambiré 2 , Kim Anh Lê 3 , Thanh Van Phan 4 , Srinivasan Velusamy 1 , Hien Anh Nguyen 3 , Dai Vo Thi Trang 4 , Fernanda C Lessa 1 , Makiko Iijima 5 , Dac Trung Nguyen 3 , Stephanie B Schwartz 1 , Lesley McGee 1 , Rasmata Ouédraogo Traoré 2 , Bernard Beall 1
Affiliation
The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.
中文翻译：
三链直接定量聚合酶链反应鉴定肺炎链球菌血清型
本研究中提出的定量聚合酶链反应 (qPCR) 方法允许在不首先进行 DNA 提取的情况下鉴定脑脊液中的肺炎球菌包膜血清型。当与提取 DNA 的标准 qPCR 方法并排比较时，这种节省时间和资源的测试方法显示出相似的灵敏度和循环阈值之间的高度一致性。
更新日期：2021-01-26
中文翻译：
三链直接定量聚合酶链反应鉴定肺炎链球菌血清型
本研究中提出的定量聚合酶链反应 (qPCR) 方法允许在不首先进行 DNA 提取的情况下鉴定脑脊液中的肺炎球菌包膜血清型。当与提取 DNA 的标准 qPCR 方法并排比较时，这种节省时间和资源的测试方法显示出相似的灵敏度和循环阈值之间的高度一致性。
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