Systemic lupus erythematosus (SLE) is a chronic systemic autoimmune disease characterized by generation of autoantibodies and severe damage of various organs.

The hormonal changes associated with pregnancy and especially estrogen might lead to damage of reproductive function and ovarian quality.

We employed a pristane-induced lupus model of Balb/c mice which resembles human lupus in an attempt to follow oogenesis disruption during the disease progression. The integrity of cytoskeletal and chromatin structures was estimated in oocytes derived by hormonally stimulated ovulation in lupus mice and the results were compared with those from healthy mice.

Chromatin, tubulin and actin structures in oocytes were detected by Hoechst 33258, anti-alpha-tubulin antibody and rhodamine-labeled phalloidin, respectively. All available meiotic spindles were analyzed – in immature (metaphase I) and mature oocytes (metaphase II).

The total number of mature oocytes obtained from lupus mice was lower compared to healthy controls. The maturation rate was 9.8 % for lupus mice, 12.7 % for 7-month old controls, and 14.3 % for the young control mice (4 weeks old). Another major difference between the studied groups was the higher percentage of defective metaphase I spindles registered in oocytes derived from lupus mice (60 % normal spindles), while for the young and older controls this proportion was 86 % and 81 %, respectively. No such difference was registered for metaphase II spindles. For both metaphase I and metaphase II oocytes, the proportions of normal actin cap and chromosomal condensation were similar between the experimental groups.

系统性红斑狼疮（SLE）是一种慢性系统性自身免疫性疾病，以自身抗体的产生和各种器官的严重损害为特征。

与怀孕相关的荷尔蒙变化，尤其是雌激素，可能会导致生殖功能和卵巢质量受损。

我们采用了一种类似于人类狼疮的 Balb/c 小鼠的 pristane 诱导的狼疮模型，试图在疾病进展过程中跟踪卵子发生中断。在狼疮小鼠激素刺激排卵衍生的卵母细胞中估计细胞骨架和染色质结构的完整性，并将结果与​​健康小鼠的结果进行比较。

Hoechst 33258、抗α-微管蛋白抗体和罗丹明标记的鬼笔环肽分别检测卵母细胞中的染色质、微管蛋白和肌动蛋白结构。分析了所有可用的减数分裂纺锤体——在未成熟（中期 I）和成熟卵母细胞（中期 II）中。

从狼疮小鼠获得的成熟卵母细胞总数低于健康对照组。狼疮小鼠的成熟率为 9.8%，7 个月大的对照为 12.7%，年轻对照小鼠（4 周大）为 14.3%。研究组之间的另一个主要差异是狼疮小鼠卵母细胞中记录的有缺陷的中期 I 纺锤体百分比较高（60% 正常纺锤体），而对于年轻和年长的对照组，这一比例分别为 86% 和 81%。中期 II 纺锤体没有这种差异。对于中期 I 和中期 II 卵母细胞，实验组之间正常肌动蛋白帽和染色体凝聚的比例相似。