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Impact of glgA1, glgA2 or glgC overexpression on growth and glycogen production in Synechocystis sp. PCC 6803
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2021-09-02 , DOI: 10.1016/j.jbiotec.2021.08.012
Sandra Mittermair 1 , Gergely Lakatos 2 , Cecilia Nicoletti 1 , Karolína Ranglová 2 , João Câmara Manoel 2 , Tomáš Grivalský 2 , Daniyar Malikuly Kozhan 3 , Jiří Masojídek 2 , Juliane Richter 1
Affiliation  

Low production rates are still one limiting factor for the industrial climate-neutral production of biovaluable compounds in cyanobacteria. Next to optimized cultivation conditions, new production strategies are required. Hence, the use of established molecular tools could lead to increased product yields in the cyanobacterial model organism Synechocystis sp. PCC6803. Its main storage compound glycogen was chosen to be increased by the use of these tools. In this study, the three genes glgC, glgA1 and glgA2, which are part of the glycogen synthesis pathway, were combined with the Pcpc560 promoter and the neutral cloning site NSC1. The complete genome integration, protein formation, biomass production and glycogen accumulation were determined to select the most productive transformants. The overexpression of glgA2 did not increase the biomass or glycogen production in short-term trials compared to the other two genes but caused transformants death in long-term trials. The transformants glgA1_11 and glgC_2 showed significantly increased biomass (1.6-fold - 1.7-fold) and glycogen production (3.5-fold - 4-fold) compared to the wild type after 96 h making them a promising energy source for further applications. Those could include for example a two-stage production process, with first energy production (glycogen) and second increased product formation (e.g. ethanol).



中文翻译:

glgA1、glgA2 或 glgC 过表达对集胞藻生长和糖原产生的影响。PCC 6803

低生产率仍然是蓝藻中生物价值化合物的工业气候中性生产的一个限制因素。除了优化栽培条件外,还需要新的生产策略。因此,使用已建立的分子工具可能会导致蓝藻模式生物集胞藻的产品产量增加。PCC6803。它的主要储存化合物糖原被选择通过使用这些工具来增加。在本研究中,作为糖原合成途径一部分的三个基因glgCglgA1glgA2与 P cpc560结合启动子和中性克隆位点 NSC1。确定完整的基因组整合、蛋白质形成、生物量产生和糖原积累以选择最具生产力的转化体。与其他两种基因相比,glgA2的过表达在短期试验中不会增加生物量或糖原产量,但在长期试验中会导致转化体死亡。转化体glgA1_11glgC_2 与野生型相比,在 96 小时后显示出显着增加的生物量(1.6 倍 - 1.7 倍)和糖原产量(3.5 倍 - 4 倍),使其成为进一步应用的有希望的能源。例如,这些可能包括两阶段生产过程,首先产生能量(糖原),第二次增加产物形成(例如乙醇)。

更新日期:2021-09-14
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