Colorimetry is widely used in assay systems for its low-cost, ease-of-use, rapidity, moderate storage requirements and intuitively visible effects. However, the application is limited due to its relatively low sensitivity. Conventional colorimetric calibration methods often use a fixed incubation time that can limit the detection range, system robustness and sensitivity. In this paper, we used color saturation to measure the accumulation of product (correlation coefficient R² = 0.9872), and we created a novel “calibration mesh” method based on an expanded sigmoid function to enhance sensitivity. The novel calibration mesh method can be adapted for a wide variety of assay systems to improve robustness and detection range, and provide a dynamic and faster output.

中文翻译：

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一种基于表面的校准方法，可实现比色测定系统的动态和准确定量

比色法因其低成本、易于使用、快速、适中的存储要求和直观可见的效果而广泛用于分析系统。然而，由于其相对较低的灵敏度，应用受到限制。传统的比色校准方法通常使用固定的孵育时间，这会限制检测范围、系统稳健性和灵敏度。在本文中，我们使用颜色饱和度来衡量积的累积（相关系数R ² = 0.9872），并且我们创建了一种基于扩展 sigmoid 函数的新型“校准网格”方法来增强灵敏度。新颖的校准网格方法可适用于多种分析系统，以提高稳定性和检测范围，并提供动态和更快的输出。