Saccharides are one of the most important biomoleculars in the world, which are responsible for various roles in living organisms. In this work, a combined use of mass spectrometry (MS) together with supercritical fluid chromatography (SFC) was applied for the separation of eight saccharides. A satisfactory separation was achieved within 15 min on a Unitary Diol column using 10 mM ammonium acetate in methanol as mobile phase additive at a temperature of 60°C. Compared to liquid chromatography–MS (LC–MS), the SFC–MS presented faster running time and better selectivity. The SFC–MS was validated and applied to the analysis of three saccharides (fructose, glucose and sucrose) present in different honey samples. The limits of detection (LODs) and limits of quantification (LOQs) of SFC–MS method for fructose, glucose and sucrose were 5.2, 4.5, 7.4 μg/mL and 17.2, 14.9, 24.1 μg/mL, respectively. The relative standard deviations for intrabatch and interbatch precision were <2.5% and the recoveries ranged from 97.6% to 103.1% for three concentration levels. The values of LODs and LOQs using SFC–MS method were lower (1.5–2.4-fold) than using LC–MS method. The results demonstrated the potential of SFC–MS for fast and sensitive determination of saccharides in honey.

中文翻译：

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超临界流体色谱与单四极杆质谱联用测定蜂蜜中的糖类

糖类是世界上最重要的生物分子之一，在生物体中发挥着各种作用。在这项工作中，质谱 (MS) 与超临界流体色谱 (SFC) 相结合用于八种糖类的分离。在 60°C 的温度下，使用 10 mM 乙酸铵的甲醇溶液作为流动相添加剂，在 Unitary Diol 色谱柱上 15 分钟内实现了令人满意的分离。与液相色谱-MS (LC-MS) 相比，SFC-MS 具有更快的运行时间和更好的选择性。SFC-MS 经验证并应用于分析不同蜂蜜样品中存在的三种糖类（果糖、葡萄糖和蔗糖）。SFC-MS 方法对果糖、葡萄糖和蔗糖的检测限 (LOD) 和定量限 (LOQ) 分别为 5.2、4.5、7。分别为 4 μg/mL 和 17.2、14.9、24.1 μg/mL。批内和批间精密度的相对标准偏差小于 2.5%，三个浓度水平的回收率范围为 97.6% 至 103.1%。使用 SFC-MS 方法的 LOD 和 LOQ 值低于使用 LC-MS 方法的值（1.5-2.4 倍）。结果证明了 SFC-MS 在快速、灵敏地测定蜂蜜中糖类方面的潜力。