Abstract

A novel nano-floral inter-polymeric material (λ-Carr-Ag-SA) was developed to effectively capture N-linked glycopeptides from biological samples. A simple one-step reduction of silver ions was involved in the synthesis of the material in which λ-carrageenan and sodium alginate, with abundant hydrophilic groups, were used as the reducing agent and stabilizer. The performance of λ-Carr-Ag-SA was evaluated experimentally by enriching glycopeptides from horse radish peroxidase (HRP) and human serum. All the results demonstrated that λ-Carr-Ag-SA had a great selectivity, low detection limit and a large binding capacity toward glycopeptides. The successful application exhibits great potential of λ-Carr-Ag-SA for further glycoproteome analysis.

摘要

开发了一种新型的纳米花卉互聚材料 (λ-Carr-Ag-SA) 以有效地从生物样品中捕获 N-连接糖肽。该材料的合成过程涉及银离子的简单一步还原，其中具有丰富亲水基团的 λ-角叉菜胶和海藻酸钠作为还原剂和稳定剂。通过从辣根过氧化物酶 (HRP) 和人血清中富集糖肽，对 λ-Carr-Ag-SA 的性能进行了实验评估。所有结果表明，λ-Carr-Ag-SA对糖肽具有较高的选择性、较低的检测限和较大的结合能力。成功的应用展示了 λ-Carr-Ag-SA 在进一步糖蛋白组分析中的巨大潜力。