Recent studies proposed that enzyme-active sites induce evolutionary constraints at long distances. The physical origin of such long-range evolutionary coupling is unknown. Here, I use a recent biophysical model of evolution to study the relationship between physical and evolutionary couplings on a diverse data set of monomeric enzymes. I show that evolutionary coupling is not universally long-range. Rather, range varies widely among enzymes, from 2 to 20 Å. Furthermore, the evolutionary coupling range of an enzyme does not inform on the underlying physical coupling, which is short range for all enzymes. Rather, evolutionary coupling range is determined by functional selection pressure.

最近的研究表明，酶活性位点会在长距离上引起进化限制。这种长程进化耦合的物理起源尚不清楚。在这里，我使用最近的进化生物物理模型来研究不同单体酶数据集上物理和进化耦合之间的关系。我表明进化耦合并不是普遍的长期耦合。相反，酶的范围差异很大，从 2 到 20 Å。此外，酶的进化耦合范围并不能说明潜在的物理耦合，这对于所有酶来说都是短距离的。相反，进化耦合范围由功能选择压力决定。