Culicoides biting midges are vectors of many haemosporidian parasite species. These parasites are found in several wild and domestic avian species in Thailand but knowledge of the insect vectors is very limited. In this study, a molecular approach was used to detect haemosporidian parasites in six biting midge species in Thailand. A total of fifteen cytochrome b haplotypes of three haemosporidian parasites were detected from 1,165 specimens of six different Culicoides species. Comparisons of these sequences with those recorded in a public database revealed that they were comprised sequences of three species, Leucocytozoon sp., Plasmodium juxtanucleare and P. gallinaceum. All of these haemosporidian parasite species were detected in Culicoides mahasarakhamense Pramual, Jomkumsing, Piraonapicha, & Jumpato while P. juxtanucleare was also detected in C. huffi Causey and C. guttifer Meijere and Leucocytozoon sp. were also detected in C. guttifer. All of these parasites are commonly found in chickens in agreement with information that these biting midge species will bite chickens. Detection DNA of Leucocytozoon sp. in biting midges reported here provides the second record of this parasitic genus infecting Culicoides. This study also provides the first records of P. juxtanucleare and P. gallinaceum in biting midge species. Further investigation is required to determine whether Culicoides biting midge species are competent vectors of these parasites.

库蠓蠓很多haemosporidian寄生虫种类的载体。这些寄生虫存在于泰国的几种野生和家养鸟类中，但对昆虫载体的了解非常有限。在这项研究中，使用分子方法检测泰国六种叮咬蚊子中的血孢子虫寄生虫。从 6 种不同库利科动物的1,165 份标本中检测到三种血孢子虫寄生虫的 15 种细胞色素b单倍型。将这些序列与公共数据库中记录的序列进行比较显示，它们由三个物种的序列组成，即白细胞虫属、近核疟原虫和P. gallinaceum. 所有这些haemosporidian寄生虫种类中检测出库蠓mahasarakhamense Pramual，Jomkumsing，Piraonapicha，与Jumpato而P. juxtanucleare中也检测C. huffi考西和C. guttifer Meijere和住白细胞SP。在C. guttifer中也检测到。所有这些寄生虫在鸡中都很常见，这与这些叮咬蚊会叮咬鸡的信息一致。Leucocytozoon sp. 的检测 DNA 。此处报道的叮咬蠓提供了该寄生属感染库蚊的第二项记录。本研究还提供了P. juxtanucleare的第一批记录和P. gallinaceum在咬蠓物种中。需要进一步研究，以确定是否库蠓蠓物种是这些寄生虫的能力的载体。