Abstract

This paper reports the chemical compounds and antimicrobial activity of essential oils isolated from the leaves, stem, and rhizomes of Alpinia kwangsiensis T. L. Wu and S. J. Chen (Zingiberaceae). The essential oils were separately isolated using hydrodistillation of the pulverized samples (2 kg each) materials in an all- glass Clevenger-type apparatus and characterized by GC-FID and GC/MS. The yields of the essential oils were 0.16, 0.11 %, and 0.21 % (w/w), respectively, calculated on a dry weight basis. The main constituents of the essential oils (percentages, respectively) were 1,8-cineole (16.9 %, 14.3 % and 5.8 %), terpinene-4-ol (16.0 %, 13.6 % and 14.3 %), (E)-methyl cinnamate (9.4 %, 15.4 % and 4.6 %) and β-pinene (7.6 %, 9.9.% and 3.1 %). The study found out that the rhizome essential oil was the most active against Enterococcus faecalis ATCC 299212 with a minimum inhibitory concentration (MIC) value of 1.60 μg/mL, while the leaf oil displayed pronounce activity towards Bacillus cereus ATCC 14579 (MIC 3.20 μg/mL). All the essential oils inhibited the growth of Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231, with MIC value of 6.40 μg/mL. Therefore, the essential oils obtained from A. kwangisensis may have potential applications as antimicrobial agents.

摘要

本文报道了从广西山姜属TL Wu 和 SJ Chen（姜科）的叶、茎和根茎中分离出的精油的化学成分和抗菌活性。在全玻璃 Clevenger 型装置中对粉碎的样品（每个 2 kg）材料进行水蒸馏，分别分离精油，并通过 GC-FID 和 GC/MS 进行表征。按干重计算，精油的产量分别为 0.16、0.11% 和 0.21% (w/w)。精油的主要成分（分别为百分比）为 1,8-桉树脑（16.9 %、14.3 % 和 5.8 %）、萜品烯-4-醇（16.0 %、13.6 % 和 14.3 %），（E)-肉桂酸甲酯（9.4%、15.4%和4.6%）和β-蒎烯（7.6%、9.9.%和3.1%）。研究发现，根茎精油对粪肠球菌ATCC 299212 的活性最强，最低抑菌浓度 (MIC) 值为 1.60 μg/mL，而叶油对蜡状芽孢杆菌ATCC 14579 (MIC 3.20 μg/ mL) 具有显着活性。毫升）。所有精油均抑制金黄色葡萄球菌ATCC 25923和白色念珠菌ATCC 10231的生长，MIC值为6.40 μg/mL。因此，从A. kwangisensis获得的精油可能具有作为抗菌剂的潜在应用。