Sofosbuvir is a direct-acting antiviral drug that inhibits hepatitis C virus (HCV) NS5B polymerase, which in turn affects the virus replication inside biological systems. The clinical importance of sofosbuvir is based not only on its effect on HCV but also on other lethal viruses such as Zika and severe acute respiratory syndrome coronavirus disease 2019 (SARS-COVID-19). Accordingly, there is a continuous shedding of light on the development and validation of accurate and fast analytical methods for the determination of sofosbuvir in different environments. This work critically reviews the recent advances in chromatographic methods for the analysis of sofosbuvir and/or its metabolites in pure samples, pharmaceutical dosage forms, and in the presence of other co-administered drugs to highlight the current status and future perspectives to enhance its determination in different matrixes.

中文翻译：

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人血浆中最常用的抗丙型肝炎药物索非布韦及其合用药物的色谱测定研究进展

Sofosbuvir 是一种直接作用的抗病毒药物，可抑制丙型肝炎病毒 (HCV) NS5B 聚合酶，进而影响生物系统内的病毒复制。索非布韦的临床重要性不仅基于其对 HCV 的影响，还基于其他致命病毒，如寨卡病毒和 2019 年严重急性呼吸综合征冠状病毒病 (SARS-COVID-19)。因此，在开发和验证用于在不同环境中测定索非布韦的准确和快速的分析方法方面，不断有新的发现。这项工作批判性地回顾了色谱方法的最新进展，用于分析纯样品、药物剂型和其他共同给药药物中的索非布韦和/或其代谢物，以突出当前状态和未来前景，以加强其测定在不同的矩阵中。