CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types.

中文翻译：

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小鼠干细胞探针 CDy1 为 ALDH2 和 Abcb1b 通过活细胞亲和矩阵和 ABC CRISPRa 文库的靶标鉴定

CDy1 是区分胚胎干细胞用于重编程研究和再生医学的有力工具。然而，CDy1 的干细胞选择性机制尚未完全了解。在这里，我们报告 ALDH2 和 ABCB1 作为 CDy1 的分子靶标，通过活细胞亲和基质和 ABC 转运蛋白 CRISPRa 库筛选阐明。两种独特的正交机制提供了特定细胞类型的多维细胞区分的潜力。