当前位置: X-MOL 学术J. Exp. Bot. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Chlamydomonas proteases: classification, phylogeny, and molecular mechanisms
Journal of Experimental Botany ( IF 6.9 ) Pub Date : 2021-08-14 , DOI: 10.1093/jxb/erab383
Yong Zou 1 , Peter V Bozhkov 1
Affiliation  

Proteases can regulate myriad biochemical pathways by digesting or processing target proteins. While up to 3% of eukaryotic genes encode proteases, only a tiny fraction of proteases are mechanistically understood. Furthermore, most of the current knowledge about proteases is derived from studies of a few model organisms, including Arabidopsis thaliana in the case of plants. Proteases in other plant model systems are largely unexplored territory, limiting our mechanistic comprehension of post-translational regulation in plants and hampering integrated understanding of how proteolysis evolved. We argue that the unicellular green alga Chlamydomonas reinhardtii has a number of technical and biological advantages for systematic studies of proteases, including reduced complexity of many protease families and ease of cell phenotyping. With this end in view, we share a genome-wide inventory of proteolytic enzymes in Chlamydomonas, compare the protease degradomes of Chlamydomonas and Arabidopsis, and consider the phylogenetic relatedness of Chlamydomonas proteases to major taxonomic groups. Finally, we summarize the current knowledge of the biochemical regulation and physiological roles of proteases in this algal model. We anticipate that our survey will promote and streamline future research on Chlamydomonas proteases, generating new insights into proteolytic mechanisms and the evolution of digestive and limited proteolysis.

中文翻译:

衣藻蛋白酶:分类、系统发育和分子机制

蛋白酶可以通过消化或加工目标蛋白质来调节无数的生化途径。虽然高达 3% 的真核基因编码蛋白酶,但只有一小部分蛋白酶被机械地理解。此外,目前关于蛋白酶的大部分知识来自对一些模式生物的研究,包括植物中的拟南芥。其他植物模型系统中的蛋白酶在很大程度上是未开发的领域,限制了我们对植物翻译后调控的机械理解,并阻碍了对蛋白水解如何进化的综合理解。我们认为单细胞绿藻莱茵衣藻对于蛋白酶的系统研究具有许多技术和生物学优势,包括降低许多蛋白酶家族的复杂性和易于细胞表型分析。鉴于此,我们分享了衣藻中蛋白水解酶的全基因组清单,比较了衣藻和拟南芥的蛋白酶降解组,并考虑了衣藻蛋白酶与主要分类群的系统发育相关性。最后,我们总结了目前关于该藻类模型中蛋白酶的生化调节和生理作用的知识。我们预计,我们的调查将促进和简化对衣藻蛋白酶的未来研究,对蛋白水解机制以及消化和有限蛋白水解的演变产生新的见解。我们总结了该藻类模型中蛋白酶的生化调节和生理作用的当前知识。我们预计,我们的调查将促进和简化对衣藻蛋白酶的未来研究,对蛋白水解机制以及消化和有限蛋白水解的演变产生新的见解。我们总结了该藻类模型中蛋白酶的生化调节和生理作用的当前知识。我们预计,我们的调查将促进和简化对衣藻蛋白酶的未来研究,对蛋白水解机制以及消化和有限蛋白水解的演变产生新的见解。
更新日期:2021-08-14
down
wechat
bug