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Design of a Hand-Held and Battery-Operated Digital Microfluidics Device Using EWOD for Lab-on-a-Chip Applications
Micromachines ( IF 3.4 ) Pub Date : 2021-09-01 , DOI: 10.3390/mi12091065
Nicholas Grant 1 , Brian Geiss 2 , Stuart Field 3 , August Demann 3 , Thomas W Chen 1, 4
Affiliation  

Microfluidics offer many advantages to Point of Care (POC) devices through lower reagent use and smaller size. Additionally, POC devices offer the unique potential to conduct tests outside of the laboratory. In particular, Electro-wetting on Dielectric (EWOD) microfluidics has been shown to be an effective way to move and mix liquids enabling many PoC devices. However, much of the research surrounding these microfluidic systems are focused on a single aspect of the system capability, such as droplet control or a specific new application at the device level using the EWOD technology. Often in these experiments the supporting systems required for operation are bench top equipment such as function generators, power supplies, and personal computers. Although various aspects of how an EWOD device is capable of moving and mixing droplets have been demonstrated at various levels, a complete self-contained and portable lab-on-a-chip system based on the EWOD technology has not been well demonstrated. For instance, EWOD systems tend to use high voltage alternating current (AC) signals to actuate electrodes, but little consideration is given to circuitry size or power consumption of such components to make the entire system portable. This paper demonstrates the feasibility of integrating all supporting hardware and software to correctly operate an EWOD device in a completely self-contained and battery-powered handheld unit. We present results that demonstrate a complete sample preparation flow for deoxyribonucleic acid (DNA) extraction and isolation. The device was designed to be a field deployable, hand-held platform capable of performing many other sample preparation tasks automatically. Liquids are transported using EWOD and controlled via a programmable microprocessor. The programmable nature of the device allows it to be configured for a variety of tests for different applications. Many considerations were given towards power consumption, size, and system complexity which make it ideal for use in a mobile environment. The results presented in this paper show a promising step forward to the portable capability of microfluidic devices based on the EWOD technology.

中文翻译:

使用 EWOD 设计用于芯片实验室应用的手持式和电池供电数字微流体装置

微流体通过更少的试剂使用和更小的尺寸为护理点 (POC) 设备提供了许多优势。此外,POC 设备具有在实验室外进行测试的独特潜力。特别是,电介质上的电润湿 (EWOD) 微流体已被证明是一种移动和混合液体的有效方法,可实现许多 PoC 设备。然而,围绕这些微流体系统的大部分研究都集中在系统能力的一个方面,例如液滴控制或使用 EWOD 技术的设备级别的特定新应用。在这些实验中,操作所需的支持系统通常是台式设备,例如函数发生器、电源和个人计算机。尽管 EWOD 设备如何能够移动和混合液滴的各个方面已经在各个层面得到了证明,但基于 EWOD 技术的完整的独立便携式芯片实验室系统还没有得到很好的证明。例如,EWOD 系统倾向于使用高压交流 (AC) 信号来驱动电极,但很少考虑此类组件的电路尺寸或功耗,以使整个系统便于携带。本文展示了集成所有支持硬件和软件以在完全独立且电池供电的手持设备中正确操作 EWOD 设备的可行性。我们提供的结果证明了脱氧核糖核酸 (DNA) 提取和分离的完整样品制备流程。该设备被设计为可现场部署的,能够自动执行许多其他样品制备任务的手持式平台。液体使用 EWOD 进行运输,并通过可编程微处理器进行控制。该器件的可编程特性使其可以针对不同应用的各种测试进行配置。对功耗、尺寸和系统复杂性进行了许多考虑,使其非常适合在移动环境中使用。本文中提出的结果表明,基于 EWOD 技术的微流体设备的便携能力向前迈出了有希望的一步。该器件的可编程特性使其可以针对不同应用的各种测试进行配置。对功耗、尺寸和系统复杂性进行了许多考虑,使其非常适合在移动环境中使用。本文中提出的结果表明,基于 EWOD 技术的微流体设备的便携能力向前迈出了有希望的一步。该器件的可编程特性使其可以针对不同应用的各种测试进行配置。对功耗、尺寸和系统复杂性进行了许多考虑,使其非常适合在移动环境中使用。本文中提出的结果表明,基于 EWOD 技术的微流体设备的便携能力向前迈出了有希望的一步。
更新日期:2021-09-01
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