The pig is the most widely used large animal model in Europe, with cardiovascular research being one of the main areas of application. Adequate refinement of interventional studies in this field, meeting the requirements of Russel and Burchs’ 3 R concept, can only be performed if blood-contacting medical devices are hemocompatible. Because most medical devices for cardiovascular interventional procedures are developed for humans they are tested mostly for compatibility with human blood. The aim of this study was therefore to determine whether there are differences in behavior of porcine and human platelets when they come into contact with glass, which was used as an exemplary thrombogenic material. For this purpose changes of platelet count, platelet volume and platelet expression of the activation markers CD61, CD62P and CD63 were measured using a modified chandler loop-system simulating the fluidic effects of the blood flow. Minipig and human platelets showed significant differences in number and volume, but not in activation after 4–8 min exposure to glass.

中文翻译：

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硼硅玻璃珠在改良钱德勒环系统中诱导的人和小型猪血小板数量、体积和活化的差异

猪是欧洲使用最广泛的大型动物模型，心血管研究是主要应用领域之一。该领域介入研究的充分细化，满足 Russel 和 Burchs 3 R 概念的要求，只有在与血液接触的医疗器械具有血液相容性的情况下才能进行。因为大多数用于心血管介入手术的医疗设备都是为人类开发的，所以它们主要是为了与人类血液的相容性进行测试。因此，本研究的目的是确定猪和人血小板与玻璃接触时的行为是否存在差异，玻璃被用作示例性血栓形成材料。为此目的，血小板计数、血小板体积和活化标志物 CD61 的血小板表达发生变化，CD62P 和 CD63 是使用改进的钱德勒回路系统模拟血流的流体效应测量的。Minipig 和人血小板在数量和体积上显示出显着差异，但在暴露于玻璃 4-8 分钟后活化没有显着差异。