Epitheaflagallin (ETFG) and epitheaflagallin 3-O-gallate (ETFGg) are minor polyphenols found in black tea extract that can be enzymatically synthesized from epigallocatechin (EGC) and epigallocatechin 3-O-gallate (EGCg), respectively, in green tea extract via laccase oxidation in the presence of gallic acid. Both compounds exhibit versatile physiological functions in vivo and in vitro, including antioxidative activity, pancreatic lipase inhibition, glycosyltransferase inhibition, and inhibition of matrix metalloprotease (MMP)-1 and MMP-3 activity and synthesis. We recently succeeded in efficiently producing ETFGg from EGCg and gallic acid by using recombinant Hericium collaroides laccase (Lcc2) in a biphasic reaction system. Using this sample, we conducted an in silico survey and in vivo tests to investigate whether ETFGg can prevent cancer based on known data showing the cancer-prevention effects of EGCg, which is a major and active component in green tea extract. We conducted docking simulation studies of the inhibitory effects of ETFGg and related compounds toward MMP-2 and MMP-9, two antiapoptotic B-cell lymphoma (Bcl)-2-family proteins, and 67-kDa laminin receptor protein (67LR). The results suggested that ETFGg interacts more strongly with Bcl-2 family proteins and 67LR compared with EGCg. Data showing the effect of ETFGg on an in vivo mouse metastasis model indicated that ETFGg is a promising functional food ingredient with potential anticancer activities. Moreover, we investigated the antidiabetic effect of ETFGg in a type-2 diabetes model mouse (KK-Ay/TaJcl) but observed no significant blood glucose inhibitory effect.

中文翻译：

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Epitheaflagallin 3-O-Gallate，一种非常微量的红茶提取物成分的抗癌作用

Epitheaflagallin（ETFG）和epitheaflagallin 3- ö -gallate（ETFGg）是次要的多酚中，可以从表棓儿茶素（EGC）和表儿茶素3-被酶促合成的红茶提取物中发现ø -gallate（EGCG），分别在绿茶提取物通过在没食子酸存在下漆酶氧化。这两种化合物在体内和体外均表现出多种生理功能，包括抗氧化活性、胰脂肪酶抑制、糖基转移酶抑制以及基质金属蛋白酶 (MMP)-1 和 MMP-3 活性和合成的抑制。我们最近通过使用重组猴头菇成功地从 EGCg 和没食子酸有效地生产了ETFGg双相反应系统中的漆酶 (Lcc2)。使用此样本，我们进行了计算机模拟调查和体内测试，根据显示 EGCg（绿茶提取物中的主要活性成分）的癌症预防效果的已知数据，研究 ETFGg 是否可以预防癌症。我们对 ETFGg 和相关化合物对 MMP-2 和 MMP-9、两种抗凋亡 B 细胞淋巴瘤 (Bcl)-2 家族蛋白和 67-kDa 层粘连蛋白受体蛋白 (67LR) 的抑制作用进行了对接模拟研究。结果表明，与 EGCg 相比，ETFGg 与 Bcl-2 家族蛋白和 67LR 的相互作用更强。显示 ETFGg 对体内影响的数据小鼠转移模型表明 ETFGg 是一种很有前景的功能性食品成分，具有潜在的抗癌活性。此外，我们研究了 ETFGg 在 2 型糖尿病模型小鼠 (KK-Ay/TaJcl) 中的抗糖尿病作用，但没有观察到显着的血糖抑制作用。