Plasma-treated poly(dimethylsiloxane) (PDMS)-supported lipid bilayers are used as functional tools for studying cell membrane properties and as platforms for biotechnology applications. Self-spreading is a versatile method for forming lipid bilayers. However, few studies have focused on the effect of plasma treatment on self-spreading lipid bilayer formation. In this paper, we performed lipid bilayer self-spreading on a PDMS surface with different treatment times. Surface characterization of PDMS treated with different treatment times is evaluated by AFM and SEM, and the effects of plasma treatment of the PDMS surface on lipid bilayer self-spreading behavior is investigated by confocal microscopy. The front-edge velocity of lipid bilayers increases with the plasma treatment time. By theoretical analyses with the extended-DLVO modeling, we find that the most likely cause of the velocity change is the hydration repulsion energy between the PDMS surface and lipid bilayers. Moreover, the growth behavior of membrane lobes on the underlying self-spreading lipid bilayer was affected by topography changes in the PDMS surface resulting from plasma treatment. Our findings suggest that the growth of self-spreading lipid bilayers can be controlled by changing the plasma treatment time.

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不同处理时间下等离子表面处理对聚二甲基硅氧烷底物脂质双层自扩散影响的研究

等离子体处理的聚二甲基硅氧烷 (PDMS) 支持的脂质双层被用作研究细胞膜特性的功能工具和生物技术应用的平台。自扩散是形成脂双层的通用方法。然而，很少有研究关注等离子体处理对自扩散脂质双层形成的影响。在本文中，我们在不同处理时间的 PDMS 表面上进行了脂质双层自扩散。用 AFM 和 SEM 评估了用不同处理时间处理的 PDMS 的表面表征，并通过共聚焦显微镜研究了等离子体处理对脂双层自扩散行为的影响。脂质双层的前沿速度随着等离子体处理时间的增加而增加。通过扩展 DLVO 建模的理论分析，我们发现速度变化的最可能原因是 PDMS 表面和脂质双层之间的水合排斥能。此外，膜瓣在底层自扩散脂双层上的生长行为受到等离子体处理导致的 PDMS 表面形貌变化的影响。我们的研究结果表明，可以通过改变等离子体处理时间来控制自扩散脂质双层的生长。