An enzymatic glucose sensor was fabricated and integrated into a heterodyne refractometer. A heterodyne light source was incident into the sensor which the circular birefringence refractive index of sample was changed as the chemical reaction between glucose oxidase and glucose progressed. Therefore, the phase difference before and after chemical reaction could be measured by a lock-in amplifier with phase-lock technology. The optical configuration and measurement procedures indicated that the alignment of the measurement system was straightforward and that it reduced the misalignment effect. The phase stability of the proposed system was superior to 0.04 deg within 1 min. The glucose sensor can be reused 100 times while retaining favorable performance. The results indicated that the resolutions of the proposed method were ∼1.5 and 1.29 mg / dl for the glucose solution and serum-based sample (SRM 965b), respectively. The calibration curve revealed high linearity within the dynamic range of 0 to 300 mg / dl. Based on these findings, the proposed method has a potential to measure the whole blood glucose concentration for daily monitoring.

中文翻译：

---

带有外差折射计的可重复使用酶比色皿，用于体外葡萄糖浓度测量

制造了酶促葡萄糖传感器并将其集成到外差折射计中。外差光源入射到传感器中，随着葡萄糖氧化酶和葡萄糖之间化学反应的进行，样品的圆形双折射折射率发生变化。因此，可以通过具有锁相技术的锁相放大器来测量化学反应前后的相位差。光学配置和测量程序表明，测量系统的对准很简单，并且减少了未对准的影响。所提出系统的相位稳定性在 1 分钟内优于 0.04 度。葡萄糖传感器可以重复使用100次，同时保持良好的性能。结果表明，所提出方法的分辨率分别为~1.5和1。葡萄糖溶液和基于血清的样品 (SRM 965b) 分别为 29 mg/dl。校准曲线在 0 到 300 mg/dl 的动态范围内显示出高线性。基于这些发现，所提出的方法有可能测量全血葡萄糖浓度以进行日常监测。