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Hepatoprotective effect and metabonomics studies of radix gentianae in rats with acute liver injury
Pharmaceutical Biology ( IF 3.8 ) Pub Date : 2021-08-31 , DOI: 10.1080/13880209.2021.1969414
Shizhao Xu 1 , Fanli Kong 1 , Zhengwu Sun 1 , Yalin Xi 1 , Fei Qi 1 , Jianzhi Sun 1
Affiliation  

Abstract

Context

As a well-known traditional Chinese medicine for protecting the liver, the mechanism of Radix Gentianae (RG) remains unclear.

Objective

The hepatoprotective effect and metabonomics of RG were studied to explore the molecular and metabolic mechanisms of RG protecting the liver.

Materials and methods

Sprague-Dawley rats were divided into control and model group (n = 10, orally given distilled water), intervention group (4 subgroups, n = 10, prophylactically and orally given 0.63, 2.5 and 5.6 g/kg RG and 0.2 g/kg bifendatatum for 7 d). On day 7 of the intervention, all rats except the control were injected intraperitoneally with 2.5% carbon tetrachloride vegetable oil solution (1.5 mL/kg) to induce liver injury. After 24 h of carbon tetrachloride injection, rat serum and liver tissue were collected for determining AST, ALT, TNF-α, MCP-1, IL-6, SOD, MDA, GSH, and GSH-PX. Rat serum was used for analysing endogenous metabolism by UPLC-Q-TOF-MS.

Results

Different doses of RG can significantly decrease the levels of AST, ALT, TNF-α, MCP-1, IL-6 and MDA, and increase the levels of SOD, GSH, and GSH-PX in rats with liver injury (p < 0.05; TNF-α, and IL-6, p < 0.05 only at 5.6 g/kg dose). Eight biomarkers of liver injury were obtained in serum metabonomics, involving five significant metabolic pathways. RG can improve steroid biosynthesis, linoleic acid metabolism, porphyrin and chlorophyll metabolism, and fatty acid biosynthesis.

Conclusion

RG demonstrated a good ability to protect the liver and improving endogenous metabolism in rats with liver injury. This can help us understand the mechanism of RG and more clinical verifications were inspired.



中文翻译:

龙胆草对急性肝损伤大鼠的保肝作用及代谢组学研究

摘要

语境

龙胆草(RG)作为一种著名的保肝中药,其作用机制尚不清楚。

客观的

研究了RG的保肝作用和代谢组学,探讨了RG保护肝脏的分子和代谢机制。

材料和方法

Sprague-Dawley大鼠分为对照组和模型组(n  = 10,口服蒸馏水),干预组(4个亚组,n  = 10,预防性和口服给予0.63、2.5和5.6 g/kg RG和0.2 g/kg bifendatatum 7 天)。干预第7天,除对照组外,所有大鼠腹腔注射2.5%四氯化碳植物油溶液(1.5 mL/kg),诱导肝损伤。四氯化碳注射24 h后,收集大鼠血清和肝组织,测定AST、ALT、TNF-α、MCP-1、IL-6、SOD、MDA、GSH和GSH-PX。大鼠血清用于UPLC-Q-TOF-MS分析内源性代谢。

结果

不同剂量的RG可显着降低肝损伤大鼠AST、ALT、TNF-α、MCP-1、IL-6、MDA水平,升高SOD、GSH、GSH-PX水平(p  < 0.05 ) ; TNF-α 和 IL-6, 仅在 5.6 g/kg 剂量时p < 0.05)。在血清代谢组学中获得了8个肝损伤的生物标志物,涉及5个重要的代谢途径。RG可以改善类固醇生物合成、亚油酸代谢、卟啉和叶绿素代谢以及脂肪酸生物合成。

结论

RG在肝损伤大鼠中表现出良好的保护肝脏和改善内源性代谢的能力。这可以帮助我们了解 RG 的机制,并启发更多的临床验证。

更新日期:2021-09-01
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