Abstract

The aim of the study was to compare the chemical composition and biological activities of Coleus barbatus aerial part essential oil (CBAEO) and Coleus barbatus root part essential oil (CBREO). The hydrodistilled essential oils (EOs) were analysed by gas chromatography-mass spectrometry (GC-MS) and biological activities were investigated in terms of antioxidant, in vitro anti-inflammatory, herbicidal, antibacterial, antifungal and antifeedant properties. GC-MS analysis led to the identification of 45 and 59 components contributing 86.7 % and 85.8 % in CBAEO and CBREO respectively. CBAEO was dominated by oxygenated monoterpenes (31.1 %) while CBREO was dominated by monoterpene hydrocarbons (29.1 %). The EOs exhibited good to moderate antioxidant potential assayed by DPPH radical, hydroxyl radical, nitric oxide radical and superoxide radical scavenging, reducing power and metal chelating activity. CBREO showed good anti-inflammatory activity (76.9 %) than CBAEO (64.7 %). Both the EOs exhibited moderate herbicidal potential against Raphanus raphanistrum subsp. sativus seeds. CBREO was observed to exhibit more antibacterial efficacy than CBAEO against Bacillus cereus and Escherichia coli. Both EOs exhibited significant antifungal activity against Alternaria alternata and Curvularia lunata and a strong antifeedant activity against third instar larvae of Spilosoma obliqua. The results obtained revealed strong to moderate efficacy of CBAEO and CBREO, which might serve as a bioactive source of natural antioxidants and an anti-inflammatory agent. The significant herbicidal, antimicrobial and anti-feedant activities of both the EOs might be helpful for the development of environmentally benign herbicides, fungicides and natural anti-feedant that could be an alternative of synthetic chemicals in future after proper clinical trials.

摘要

该研究的目的是比较的化学成分和生物活性彩叶石竹地上部分精油（CBAEO）和彩叶石竹根部精油（CBREO）。通过气相色谱-质谱法 (GC-MS) 分析水蒸馏精油 (EOs) 并在体外研究抗氧化方面的生物活性抗炎、除草、抗菌、抗真菌和拒食特性。GC-MS 分析鉴定了 45 和 59 种成分，分别占 CBAEO 和 CBREO 的 86.7% 和 85.8%。CBAEO 以含氧单萜（31.1%）为主，而 CBREO 以单萜烃（29.1%）为主。通过DPPH自由基、羟基自由基、一氧化氮自由基和超氧自由基清除、还原能力和金属螯合活性测定，EOs表现出良好至中等的抗氧化潜力。CBREO 显示出比 CBAEO (64.7%) 更好的抗炎活性 (76.9%)。两种 EO 都对Raphanus raphanistrum subsp表现出中等除草潜力。苜蓿种子。观察到 CBREO 表现出比 CBAEO 更强的抗菌功效蜡样芽孢杆菌和大肠杆菌。两种 EOs 对Alternaria alternata和Curvularia lunata均表现出显着的抗真菌活性，对Spilosomaobliqua 的三龄幼虫表现出很强的拒食活性。获得的结果揭示了 CBAEO 和 CBREO 的强至中等功效，它们可能作为天然抗氧化剂和抗炎剂的生物活性来源。两种 EO 的显着除草、抗菌和抗拒食​​活性可能有助于开发环境友好的除草剂、杀菌剂和天然拒食剂，在适当的临床试验后，它们可能在未来成为合成化学品的替代品。