In this work supercritical fluids extraction (SFE) was applied on Schizochytrium algae biomass; the main focus was the application of different pretreatments and to study the influence of the pressure and temperature on lipids yields and docosahexaenoic acid (DHA) concentration. The optimum conditions were obtained at 46.52 MPa and 76.85 °C using ethanol as a co-solvent (1.25 mL/min). After 30 min, 19.40 g of lipids/100 g of dry biomass and 7.98 g of DHA/100 g of dry biomass (45%) were produced. Grinding as pretreatment increased extraction efficiency from 30% to 76% and compared with other methods with solvents, obtained 34.29 g of lipids/100 g of dry biomass, and 17.51 g of DHA/100 g of dry biomass (51%). Our data show that the SFE with pretreatment allowed the extraction of high lipid yields and DHA concentrations in shorter extraction times.

在这项工作中，超临界流体萃取 (SFE) 应用于裂殖壶菌藻类生物量；主要重点是应用不同的预处理，并研究压力和温度对脂质产量和二十二碳六烯酸 (DHA) 浓度的影响。最佳条件是在 46.52 MPa 和 76.85 °C 下获得，使用乙醇作为共溶剂 (1.25 mL/min)。30 分钟后，产生了 19.40 克脂质/100 克干生物质和 7.98 克 DHA/100 克干生物质（45%）。研磨作为预处理将提取效率从 30% 提高到 76%，与使用溶剂的其他方法相比，获得 34.29 g 脂质/100 g 干生物质和 17.51 g DHA/100 g 干生物质（51%）。我们的数据表明，经过预处理的 SFE 允许在更短的提取时间内提取高脂质产量和 DHA 浓度。