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The Sur7 cytoplasmic C terminus regulates morphogenesis and stress responses in Candida albicans
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-08-31 , DOI: 10.1111/mmi.14806
Carla E Lanze 1 , Sai Zhou 1 , James B Konopka 1
Affiliation  

MCC/eisosome subdomains of the plasma membrane promote proper cell wall morphogenesis that is critical for the fungal pathogen Candida albicans to grow invasively and resist stressful environments in the host. Sur7 localizes to MCC/eisosomes and is needed for their function, so in this work, the role of this tetraspan membrane protein was studied by mutagenesis. Deletion mutant analysis showed that the N-terminal region containing the four transmembrane domains mediates Sur7 localization to MCC/eisosomes. Mutation of 32 conserved residues in the N-terminal region indicated that extracellular loop 1 is important, although these mutants generally displayed weak phenotypes. Surprisingly, two Cys residues in a conserved motif in extracellular loop 1 were not important. However, deletion of the entire 15 amino acid motif revealed that it was needed for proper membrane trafficking of Sur7. Deletion and substitution mutagenesis showed that the C terminus is important for resisting cell wall stress. This is significant as it indicates Sur7 carries out an important role in the cytoplasm. Altogether, these results indicate that the N-terminal region localizes Sur7 to MCC/eisosomes and that the C-terminal domain promotes responses in the cytoplasm needed for cell wall morphogenesis and stress resistance.

中文翻译:

Sur7 细胞质 C 末端调节白色念珠菌的形态发生和应激反应

质膜的 MCC/异构体亚结构域促进适当的细胞壁形态发生,这对真菌病原体白色念珠菌至关重要侵入性地生长并抵抗宿主的压力环境。Sur7 定位于 MCC/异构体,是其功能所必需的,因此在这项工作中,通过诱变研究了这种四跨膜蛋白的作用。缺失突变体分析表明,含有四个跨膜结构域的 N 末端区域介导 Sur7 定位到 MCC/eisosomes。N 末端区域中 32 个保守残基的突变表明细胞外环 1 很重要,尽管这些突变体通常表现出较弱的表型。令人惊讶的是,细胞外环 1 中保守基序中的两个 Cys 残基并不重要。然而,整个 15 个氨基酸基序的缺失表明 Sur7 的正确膜运输需要它。缺失和替代诱变表明C末端对于抵抗细胞壁应力很重要。这很重要,因为它表明 Sur7 在细胞质中发挥着重要作用。总之,这些结果表明,N 端区域将 Sur7 定位于 MCC/异构体,并且 C 端结构域促进了细胞壁形态发生和抗逆性所需的细胞质中的反应。
更新日期:2021-10-25
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