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Quantitative Determination of 15 Active Components in Lepidium meyenii with UHPLC-PDA and GC-MS
Journal of Analytical Methods in Chemistry ( IF 2.6 ) Pub Date : 2021-09-01 , DOI: 10.1155/2021/6333989
Yao-Qing Xu 1 , San-Yang Qiao 2 , Zi-Qian Wang 3 , Meng-Yao Cui 4 , Dao-Peng Tan 1 , Hong Feng 1 , Xing-Sha Mei 5 , Geng Li 4, 6 , Long Cheng 1
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In this study, a method using ultrahigh-performance liquid chromatography with photodiode array (UHPLC-PDA) was established and validated for the simultaneous quantification of 10 active components, including eight macamides and two glucosinolates, in Lepidium meyenii (maca). A gas chromatographic mass spectroscopy (GC-MS) method was used to determine the levels of three benzyl isothiocyanates and two sterols in maca. Liquid chromatographic separation was achieved on a Waters Acquity UHPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) with gradient elution over 15 min. The mobile phase was (B) acetonitrile-(A) 10 mM aqueous ammonium phosphate, and the detection wavelength was 210 nm. The gas chromatographic separation was performed on an SH-Rxi-1 MS column, and the ionization mode was electron ionization (EI). Two methods were confirmed to have desirable precision (RSD < 1.58%), repeatability (RSD < 1.97%), stability (RSD < 1.76%), and good linearity (R2 ≥ 0.999) within the test range. The recoveries were in the range of 96.79–109.99%, with an RSD below 2.39%. We applied the established methods and successfully analyzed 15 compounds in maca processed under different drying conditions, providing a comprehensive reference for maca processing method of development. In summary, this study provided two rapid and effective methods for the quantification of 15 active components, which contributed to the in-depth maca quality control and provided a reference for the development of maca products.

中文翻译:

使用 UHPLC-PDA 和 GC-MS 定量测定 Lepidium meyenii 中的 15 种活性成分

在本研究中,建立并验证了一种使用带光电二极管阵列的超高效液相色谱 (UHPLC-PDA) 的方法,并验证了该方法可同时定量分析Lepidium meyenii (maca) 中的 10 种活性成分,包括 8 种澳洲坚果和两种芥子油苷。使用气相色谱质谱 (GC-MS) 方法测定玛咖中三种异硫氰酸苄酯和两种甾醇的含量。液相色谱分离是在 Waters Acquity UHPLC HSS T3 柱(2.1 mm × 100 mm,1.8  μm) 梯度洗脱超过 15 分钟。流动相为(B)乙腈-(A)10 mM磷酸铵水溶液,检测波长为210 nm。气相色谱分离在SH-Rxi-1 MS色谱柱上进行,电离模式为电子电离(EI)。两种方法经证实具有理想的精密度 (RSD < 1.58%)、重复性 (RSD < 1.97%)、稳定性 (RSD < 1.76%) 和良好的线性 ( R 2 ≥ 0.999) 在测试范围内。回收率在 96.79-109.99% 之间,RSD 低于 2.39%。我们应用已建立的方法,成功分析了不同干燥条件下加工的玛咖中的15种化合物,为玛咖加工方法的开发提供了全面的参考。综上所述,本研究为15种活性成分的定量提供了两种快速有效的方法,有助于深入玛咖质量控制,为玛咖产品的开发提供参考。
更新日期:2021-09-01
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