Purpose

Common scab of potatoes (CS) is influenced by plant-microbe-soil interactions, which are locally specific. The study aims to identify any common trends in such interactions across a landscape differentiated by soil pH and CS severity.

Methods

The 33 sites and 17 cultivars were characterized using soil and periderm nutrient contents and microbial communities. Quantitative PCR and Illumina amplicon sequencing were used to assess abundance of bacteria, actinobacteria and pathogens, and community composition.

Results

Comparisons between bulk and tuberosphere soil compartments as well as potato cultivars divided to three categories of CS susceptibility revealed that nitrogen was elevated in tuberosphere soil and N, Mg and Fe were lowered in periderm of resistant cultivars. The susceptible cultivar Agria grown at 7 sites had higher Ca content in tuberosphere soil, while the resistant cultivar Adela grown at 10 sites had higher S, P and Mg contents in its tuberosphere soil and P and Fe in periderm. That suggests further interactions between plants and bacterial community involving nutrient uptake. Diversity of bacteria was positively correlated with CS severity suggesting interactions between the Streptomyces pathogen populations and the local soil community.

Conclusions

Overall, pathogen abundance assessed by quantifying the thaxtomin biosynthetic txtB genes were randomly dispersed among the sites without connections to CS severity or soil pH. Thus, the significant differences between bacterial communities of bulk and tuberosphere soils together with cultivar CS susceptibility showed that the susceptible cultivars select bacterial community relatively similar to the bulk soil, while the resistant cultivars promote more distinct communities.

中文翻译：

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不同结痂严重程度的田地中的块茎和大块土壤微生物群落通过土壤化学和与病原体的相互作用来区分

目的

常见的马铃薯疮痂病 (CS) 受植物-微生物-土壤相互作用的影响，具有局部特异性。该研究旨在确定通过土壤 pH 值和 CS 严重程度区分的景观中这种相互作用的任何共同趋势。

方法

使用土壤和周皮养分含量以及微生物群落对 33 个地点和 17 个栽培品种进行了表征。定量 PCR 和 Illumina 扩增子测序用于评估细菌、放线菌和病原体的丰度以及群落组成。

结果

大块土壤和块茎土壤隔室以及分为三类 CS 敏感性的马铃薯品种之间的比较表明，块茎土壤中的氮含量升高，而抗性品种周皮中的 N、Mg 和 Fe 含量降低。7个地点种植的感病品种Agria块茎土壤Ca含量较高，而10个地点种植的抗病品种Adela块茎土壤中S、P、Mg含量较高，周皮中P、Fe含量较高。这表明植物和细菌群落之间涉及养分吸收的进一步相互作用。细菌多样性与 CS 严重程度呈正相关，表明链霉菌病原体种群与当地土壤群落之间存在相互作用。

结论

总体而言，通过量化 thaxtomin 生物合成txtB基因评估的病原体丰度随机分散在站点中，与 CS 严重程度或土壤 pH 值无关。因此，大块土壤和块茎土壤细菌群落之间的显着差异以及品种对 CS 的敏感性表明，敏感品种选择的细菌群落与大块土壤相对相似，而抗性品种促进更不同的群落。