当前位置: X-MOL 学术Microsyst. Nanoeng. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
DMD-based hyperspectral microscopy with flexible multiline parallel scanning
Microsystems & Nanoengineering ( IF 7.9 ) Pub Date : 2021-09-01 , DOI: 10.1038/s41378-021-00299-2
Xue Dong 1, 2, 3, 4 , Geng Tong 1, 2, 3, 4 , Xuankun Song 1, 2, 3, 4 , Xingchen Xiao 1, 2, 3, 4 , Yiting Yu 1, 2, 3, 4
Affiliation  

As one of the most common hyperspectral microscopy (HSM) techniques, line-scanning HSM is currently utilized in many fields. However, its scanning efficiency is still considered to be inadequate since many biological and chemical processes occur too rapidly to be captured. Accordingly, in this work, a digital micromirror device (DMD) based on microelectromechanical systems (MEMS) is utilized to demonstrate a flexible multiline scanning HSM system. To the best of our knowledge, this is the first line-scanning HSM system in which the number of scanning lines N can be tuned by simply changing the DMD’s parallel scanning units according to diverse applications. This brilliant strategy of effortless adjustability relies only on on-chip scanning methods and totally exploits the benefits of parallelization, aiming to achieve nearly an N-time improvement in the detection efficiency and an N-time decrease in the scanning time and data volume compared with the single-line method under the same operating conditions. To validate this, we selected a few samples of different spectral wavebands to perform reflection imaging, transmission imaging, and fluorescence imaging with varying numbers of scanning lines. The results show the great potential of our DMD-based HSM system for the rapid development of cellular biology, material analysis, and so on. In addition, its on-chip scanning process eliminates the inherent microscopic architecture, making the whole system compact, lightweight, portable, and not subject to site constraints.



中文翻译:

具有灵活多线平行扫描的基于 DMD 的高光谱显微镜

作为最常见的高光谱显微镜 (HSM) 技术之一,线扫描 HSM 目前被用于许多领域。然而,它的扫描效率仍然被认为是不够的,因为许多生物和化学过程发生得太快而无法被捕获。因此,在这项工作中,利用基于微机电系统 (MEMS) 的数字微镜器件 (DMD) 来演示灵活的多线扫描 HSM 系统。据我们所知,这是第一个行扫描 HSM 系统,其中扫描线数为N可以根据不同的应用通过简单地改变 DMD 的并行扫描单元来调整。这种轻松可调的绝妙策略仅依赖于片上扫描方法,并充分利用并行化的优势,旨在实现检测效率的近N倍提高和N- 在相同操作条件下,与单线方式相比,扫描时间和数据量减少。为了验证这一点,我们选择了几个不同光谱波段的样本,用不同数量的扫描线进行反射成像、透射成像和荧光成像。结果表明,我们基于 DMD 的 HSM 系统在细胞生物学、材料分析等领域的快速发展方面具有巨大潜力。此外,其片上扫描过程消除了固有的微观架构,使整个系统紧凑、轻便、便携,不受场地限制。

更新日期:2021-09-01
down
wechat
bug