ABSTRACT

Introduction

Active matrix metalloproteinase (aMMP)-8 utilized in point-of-care testing (POCT) is regarded as a potential biomarker for periodontal and peri-implant diseases. Various host and microbial factors eventually influence the expression, degranulation, levels and activation of aMMP-8. The type of oral fluids (saliva, mouthrinse, gingival crevicular, and peri-implant sulcular fluids [GCF/PISF], respectively) affect the analysis.

Areas covered

With this background, we aimed to review here the recent studies on practical, inexpensive, noninvasive and quantitative mouthrinse and GCF/PISF chair-side POCT lateral flow aMMP-8 immunoassays (PerioSafe and ImplantSafe/ORALyzer) and how they help to detect, predict, monitor the course, treatment and prevention of periodontitis and peri-implantitis. The correlations of aMMP-8 POCT to other independent and catalytic activity assays of MMP-8 are also addressed.

Expert opinion

The mouthrinse aMMP-8 POCT can also detect prediabetes/diabetes and tissue destructive oral side-effects due to the head and neck cancers’ radiotherapy. Chlorhexidine and doxycycline can inhibit collagenolytic human neutrophil and GCF aMMP-8. Furthermore, by a set of case-series we demonstrate the potential of mouthrinse aMMP-8 POCT to real-time/online detect periodontitis as a potential risk disease for coronavirus disease 2019 (COVID-19). The clinical interdisciplinary utilization of aMMP-8 POCT requires additional oral, medical, and interdisciplinary studies.

中文翻译：

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COVID-19 大流行中的活性基质金属蛋白酶 8 (aMMP-8) 即时检验 (POCT)

摘要

介绍

用于即时检测 (POCT) 的活性基质金属蛋白酶 (aMMP)-8 被认为是牙周和种植体周围疾病的潜在生物标志物。各种宿主和微生物因素最终会影响 aMMP-8 的表达、脱粒、水平和活化。口腔液的类型（分别为唾液、漱口水、龈沟液和种植体周围龈沟液 [GCF/PISF]）会影响分析。

覆盖区域

在此背景下，我们旨在回顾最近关于实用、廉价、无创和定量漱口水和 GCF/PISF 椅侧 POCT 侧流 aMMP-8 免疫测定（PerioSafe 和 ImplantSafe/ORALyzer）的研究以及它们如何帮助检测、预测，监测牙周炎和种植体周围炎的病程、治疗和预防。还讨论了 aMMP-8 POCT 与 MMP-8 的其他独立和催化活性测定的相关性。

专家意见

漱口水 aMMP-8 POCT 还可以检测由于头颈癌放疗引起的前驱糖尿病/糖尿病和组织破坏性口腔副作用。洗必泰和强力霉素可以抑制溶胶原的人中性粒细胞和 GCF aMMP-8。此外，通过一组病例系列，我们证明了漱口水 aMMP-8 POCT 在实时/在线检测牙周炎作为 2019 年冠状病毒病 (COVID-19) 的潜在风险疾病方面的潜力。aMMP-8 POCT 的临床跨学科应用需要额外的口腔、医学和跨学科研究。