Ointments are widely used as topical medication as a mode of transdermal drug delivery for treatment of skin lesions. In this study, Tridax procumbens infused oil (oil phase) and Gelatin stabilized silver nanoparticle (Aqueous phase) were blended with natural biosurfactant extracted from Lactobacillus casei (MT012285) to prepare an ointment for topical wounds. GC–MS of infused oil showed the presence of pentadecanoic acid and 1,19-eicosadiene. FT-IR studies show the presence of aliphatic and amino group of biosurfactant from L. casei confirm the presence of lipopeptide, which can form stable W/O emulsion in ointment. The prepared ointment shows higher microbial reduction capacity on clinical pathogens tested namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Further, herbal-nano-ointment exhibits minimal hemolytic action on human red blood cells at 100 µg/mL. At higher concentration of 4.0 mg/mL of ointment low cytotoxic effect on L929 fibroblastic cell line was observed. Cell migration rate of 62% was recorded at 24 h using wound scratch assay. Based on the study we find that biosurfactant-based nano-topical ointment from T. procumbens oil with G-AgNPs, has low cytotoxic effect and a high cell migration rate and hence can be used in the topical application for treatment of wound.

软膏被广泛用作局部用药，作为治疗皮肤损伤的一种透皮给药方式。在这项研究中，Tridax procumbens注入油（油相）和明胶稳定的银纳米颗粒（水相）与从干酪乳杆菌(MT012285) 中提取的天然生物表面活性剂混合，制备用于局部伤口的软膏。注入油的 GC-MS 显示存在十五烷酸和 1,19-二十碳二烯。FT-IR 研究表明来自干酪乳杆菌的生物表面活性剂的脂肪族和氨基的存在证实了脂肽的存在，它可以在软膏中形成稳定的 W/O 乳液。制备的软膏对临床试验病原体显示出较高的微生物减少能力，即大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和肺炎克雷伯菌。此外，草药纳米软膏在 100 µg/mL 时对人类红细胞的溶血作用最小。在 4.0 mg/mL 的较高浓度下，观察到对 L929 成纤维细胞系的低细胞毒性作用。使用伤口划痕试验在 24 小时时记录了 62% 的细胞迁移率。基于该研究，我们发现具有 G-AgNPs 的T. procumbens油的基于生物表面活性剂的纳米外用软膏具有低细胞毒性和高细胞迁移率，因此可用于治疗伤口的局部应用。