Organ-on-chip (OoC) systems have become a promising tool for personalized medicine and drug development with advantages over conventional animal models and cell assays. However, the utility of OoCs in industrial settings is still limited, as external pumps and tubing for on-chip fluid transport are dependent on error-prone, manual handling. Here, we present an on-chip pump for OoC and Organ-Disc systems, to perfuse media without external pumps or tubing. Peristaltic pumping is implemented through periodic compression of a flexible pump layer. The disc-shaped, microfluidic module contains four independent systems, each lined with endothelial cells cultured under defined, peristaltic perfusion. Both cell viability and functionality were maintained over several days shown by supernatant analysis and immunostaining. Integrated, on-disc perfusion was further used for cytokine-induced cell activation with physiologic cell responses and for whole blood perfusion assays, both demonstrating the versatility of our system for OoC applications.

中文翻译：

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用于无 PDMS 芯片和器官盘系统中的可调介质循环和全血灌注的蠕动芯片泵

器官芯片 (OoC) 系统已成为个性化医疗和药物开发的一种很有前途的工具，与传统的动物模型和细胞分析相比具有优势。然而，OoC 在工业环境中的效用仍然有限，因为用于片上流体传输的外部泵和管道依赖于容易出错的手动处理。在这里，我们展示了一种用于 OoC 和 Organ-Disc 系统的片上泵，无需外部泵或管道即可灌注培养基。蠕动泵是通过周期性压缩柔性泵层来实现的。圆盘形微流体模块包含四个独立的系统，每个系统内衬在确定的蠕动灌注下培养的内皮细胞。通过上清液分析和免疫染色显示，细胞存活率和功能性都维持了几天。融合的，