Cetyl trimethyl ammonium bromide (CTAB) modified montmorillonite (MMT) clay (CTAB-MT) doped, tasar silk fibroin-polyvinyl alcohol blend-based 3D nanowebs are generated through electrospinning technique. The morphological analysis reveals the formation of interlinked 3D nanoweb-like architecture and high surface roughness through scanning electron microscopy (SEM) and atomic force microscopy, respectively. The existence of CTAB-MT in nanowebs is confirmed by Fourier transform infrared and complete exfoliation of clay in the polymer blend matrix along with the altered crystallinity of samples is indicated in X-ray diffraction. The incorporation of CTAB-MT clay has shown the enhancement of thermal and mechanical properties of nanoweb samples while the water uptake capacity is reduced and enzymatic biodegradability is found to slow down. The samples present excellent biocompatibility with no cytotoxicity in the Alamar blue assay and high attachment as well as spreading of L929 fibroblast cells covering the entire surface as observed in SEM. The CTAB-MT clay has imparted the samples with good antimicrobial activity against E. coli and S. aureus bacterial strains. The aforementioned properties of these CTAB-MT clay doped 3D nanowebs direct toward their suitability as a potential candidate for tissue engineering applications in the biomedical field.

中文翻译：

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十六烷基三甲基溴化铵改性蒙脱石掺杂 Tasar 丝素/聚乙烯醇混合物 3D 纳米网，用于组织工程应用

十六烷基三甲基溴化铵 (CTAB) 改性蒙脱石 (MMT) 粘土 (CTAB-MT) 掺杂、基于 tasar 丝素蛋白-聚乙烯醇混合物的 3D 纳米网是通过静电纺丝技术生成的。形态分析分别通过扫描电子显微镜 (SEM) 和原子力显微镜揭示了相互连接的 3D 纳米网状结构和高表面粗糙度的形成。CTAB-MT 在纳米纤维网中的存在通过傅里叶变换红外证实，聚合物共混基质中粘土的完全剥离以及样品结晶度的改变在 X 射线衍射中表明。CTAB-MT 粘土的掺入表明纳米纤维网样品的热性能和机械性能增强，同时吸水能力降低，酶促生物降解性减慢。样品在 Alamar blue 测定中表现出极好的生物相容性，没有细胞毒性，并且如在 SEM 中观察到的那样，L929 成纤维细胞的高度附着和扩散覆盖了整个表面。CTAB-MT 粘土赋予样品良好的抗微生物活性大肠杆菌和金黄色葡萄球菌菌株。这些 CTAB-MT 粘土掺杂的 3D 纳米网的上述特性直接适用于它们作为生物医学领域组织工程应用的潜在候选者。