当前位置: X-MOL 学术J. Histochem. Cytochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Improvement in Double Staining With Fluoro-Jade C and Fluorescent Immunostaining: FJC Staining Is Not Specific to Degenerating Mature Neurons
Journal of Histochemistry & Cytochemistry ( IF 3.2 ) Pub Date : 2021-08-31 , DOI: 10.1369/00221554211043340
Takuya Ikenari 1 , Tatsuya Kawaguchi 2 , Rei Ota 1 , Miki Matsui 1 , Ryota Yoshida 1 , Tetsuji Mori 1
Affiliation  

Fluoro-Jade C (FJC) staining has been used to detect degenerating neurons in tissue sections. It is a simple and easy staining procedure and does not depend on the manner of cell death. In some experiments, double staining with FJC and fluorescent immunostaining (FI) is required to identify cell types. However, pretreatment for FJC staining contains some processes that are harsh to fluorophores, and the FI signal is greatly reduced. To overcome this issue, we improved the double staining protocol to acquire clear double-stained images by introducing the labeled streptavidin–biotin system. In addition, several studies indicate that FJC can label non-degenerating glial cells, including resting/reactive astrocytes and activated microglia. Moreover, our previous study indicated that degenerating mesenchymal cells were also labeled by FJC, but it is still unclear whether FJC can label degenerating glial cells. Acute encephalopathy model mice contained damaged astrocytes with clasmatodendrosis, and 6-aminonicotinamide-injected mice contained necrotic astrocytes and oligodendrocytes. Using our improved double staining protocol with FJC and FI, we detected FJC-labeled degenerating astrocytes and oligodendrocytes with pyknotic nuclei. These results indicate that FJC is not specific to degenerating neurons in some experimental conditions:



中文翻译:

Fluoro-Jade C 和荧光免疫染色对双重染色的改进:FJC 染色并不特定于退化的成熟神经元

Fluoro-Jade C (FJC) 染色已用于检测组织切片中的退化神经元。这是一个简单易行的染色程序,不依赖于细胞死亡的方式。在一些实验中,需要使用 FJC 和荧光免疫染色 (FI) 进行双重染色来识别细胞类型。然而,FJC染色的预处理包含一些对荧光团苛刻的过程,FI信号大大降低。为了克服这个问题,我们通过引入标记的链霉亲和素-生物素系统改进了双染色方案以获得清晰的双染色图像。此外,一些研究表明 FJC 可以标记非退化的神经胶质细胞,包括静息/反应性星形胶质细胞和活化的小胶质细胞。此外,我们之前的研究表明,退化的间充质细胞也被 FJC 标记,但目前尚不清楚 FJC 是否可以标记退化的神经胶质细胞。急性脑病模型小鼠含有受损的星形胶质细胞并伴有裂状胶质细胞,注射 6-氨基烟酰胺的小鼠含有坏死的星形胶质细胞和少突胶质细胞。使用我们改进的 FJC 和 FI 双染色方案,我们检测到具有固缩核的 FJC 标记的退化星形胶质细胞和少突胶质细胞。这些结果表明,在某些实验条件下,FJC 并不是特定于退化神经元:我们检测到具有固缩核的 FJC 标记的退化星形胶质细胞和少突胶质细胞。这些结果表明,在某些实验条件下,FJC 并不是特定于退化神经元:我们检测到具有固缩核的 FJC 标记的退化星形胶质细胞和少突胶质细胞。这些结果表明,在某些实验条件下,FJC 并不是特定于退化神经元:

更新日期:2021-08-31
down
wechat
bug