Abstract

A reusable magnetic nanoparticlesbased cryogel optical biosensor was fabricated to determine of sucrose in sugar cane and sugar samples. In this design, magnetic nanoparticles were embedded in cryogel and then the multienzymes were immobilized. The magnetic nanoparticles in cryogel helped separate the immobilized multi-enzyme cryogel biosensor by applying the external magnetic field to reuse for the subsequent detection. The developed cryogel biosensor exhibited good selectivity for sucrose determination with the detection limit of 3 mM and can be reused up to 32 times with good repeatability (<5 % RSD). Furthermore, the proposed optical biosensor can also be utilized for sucrose analysis in sugar cane and sugar samples.

摘要

制造了一种可重复使用的基于磁性纳米粒子的冷冻凝胶光学生物传感器，用于测定甘蔗和糖样品中的蔗糖。在该设计中，磁性纳米颗粒嵌入冷冻凝胶中，然后固定多酶。冷冻凝胶中的磁性纳米颗粒通过施加外部磁场帮助分离固定的多酶冷冻凝胶生物传感器，以重复使用以进行后续检测。开发的冷冻凝胶生物传感器对蔗糖测定具有良好的选择性，检测限为 3 mM，可重复使用多达 32 次，具有良好的重复性（<5 % RSD）。此外，所提出的光学生物传感器还可用于甘蔗和糖样品中的蔗糖分析。