The current study investigates the antioxidant activity of Acalypha indica aerial parts and root ethanolic extracts and explore whether these extracts will stimulate fibroblasts viability and ability to migrate. Aerial parts extract exhibited higher DPPH scavenging activity compared to root extract with IC50 of 62 µg/mL and 206 µg/mL, respectively. Both aerial parts and root extracts showed low cytotoxicity towards fibroblasts with 753 µg/mL LD50 for aerial parts and undetected LD50 for root extract. Additionally, aerial parts extract significantly induces fibroblasts proliferation up to 134%. Wound closure investigation showed a significant closure percentage for aerial parts compared to untreated control with 75% at 1 µg/mL and high closure percentage with 70% at 0.1 µg/mL for root extract compared to only 59% closure percentage for untreated control after 48 h of the study. This study provided evidence for A. indica to have great wound healing potential. The finding builds the scientific background in future to utilise the high antioxidant activity of A. indica and its ability to stimulate fibroblasts migration and proliferation for further applications.

中文翻译：

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Acalypha indica 的体外成纤维细胞活力和迁移刺激：对伤口愈合活性的洞察

目前的研究调查了 Acalypha indica 地上部分和根乙醇提取物的抗氧化活性，并探讨这些提取物是否会刺激成纤维细胞的活力和迁移能力。与根提取物相比，地上部分提取物表现出更高的 DPPH 清除活性，IC50 分别为 62 µg/mL 和 206 µg/mL。地上部分和根提取物对成纤维细胞均显示出低细胞毒性，地上部分的 LD50 为 753 µg/mL，根提取物的 LD50 未检测到。此外，地上部分提取物显着诱导成纤维细胞增殖高达 134%。伤口闭合调查显示，与未经处理的对照组相比，地上部分的闭合百分比显着，1 µg/mL 时为 75%，0 时为 70% 的高闭合百分比。研究 48 小时后，根提取物的闭合百分比为 1 µg/mL，而未处理对照的闭合百分比仅为 59%。这项研究为 A. indica 具有巨大的伤口愈合潜力提供了证据。这一发现为未来利用 A. indica 的高抗氧化活性及其刺激成纤维细胞迁移和增殖的能力奠定了科学背景，以进一步应用。