The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa. Here, we describe a novel ncRNA sharing many features characteristic of 7SK RNAs, in D. melanogaster. We examined the structure of the corresponding gene and determined the expression profiles of the encoded RNA, called snRNA:7SK:94F, during development. It is probably produced from the transcription of a lncRNA which is processed into a mature snRNA. We also addressed its biological function and we show that, like dm7SK, this alternative 7SK interacts in vivo with the different partners of the P-TEFb complex, i.e. HEXIM, LARP7 and Cyclin T. This novel RNA is widely expressed across tissues. We propose that two distinct 7SK genes might contribute to the formation of the 7SK snRNP complex in D. melanogaster.

中文翻译：

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另一种黑腹果蝇 7SK snRNP

大多数后生动物中发现的 7SK 小核 RNA (snRNA) 是 P-TEFb 的关键调节因子，P-TEFb 反过来又调节 RNA 聚合酶 II 的延伸。尽管其一级序列在原口动物中有所不同，但其二级结构和功能在进化的远距离分类群中是保守的。在这里，我们描述了黑腹果蝇中一种新型 ncRNA，它具有 7SK RNA 的许多特征。我们检查了相应基因的结构，并确定了发育过程中所编码的 RNA（称为 snRNA:7SK:94F）的表达谱。它可能是由 lncRNA 转录产生的，该 lncRNA 被加工成成熟的 snRNA。我们还探讨了其生物学功能，并表明，与 dm7SK 一样，这种替代 7SK 在体内与 P-TEFb 复合物的不同伙伴（即 HEXIM、LARP7 和 Cyclin T）相互作用。这种新型 RNA 在组织中广泛表达。我们认为两个不同的 7SK 基因可能有助于黑腹果蝇 7SK snRNP 复合物的形成。