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A Novel Method for Ex-Vivo Latex Angiography of the Mouse Brain
Journal of Neuroscience Methods ( IF 3 ) Pub Date : 2021-08-31 , DOI: 10.1016/j.jneumeth.2021.109342
Keyan Peterson 1 , Stephanie Coffman 1 , Stacey Wolfe 1 , Zhidan Xiang 1
Affiliation  

Introduction

Latex perfusion is an effective tool to study cerebrovascular pathology in the animal brain. It provides, low-cost, high fidelity anatomical information on ex-vivo analysis, and can be utilized to study multiple, states. However, current methods of latex casting and tissue-clearance do not allow for, immunohistochemical analysis following sample processing. This results in experiments that require, increased numbers of animals to attain adequate data.

New method

In this paper, we present a modified latex perfusion and tissue processing protocol for ex-vivo analysis, of the cerebral vasculature. The method consists of injection of the arterial tree with liquid latex, followed by tissue clearance with a scale solution.

Results

Our results demonstrate effective and reliable perfusion of the murine cerebrovascular tree, rendering, the arterial morphology of the brain in high detail, while allowing for post-perfusion, immunohistochemistry of the sample.

Comparison with existing method

Our technique bypasses the limitations of previous latex angiography protocols by allowing for postperfusion, pathologic analysis of casted cerebrovascular tissue.

Conclusion

This protocol provides a reliable, low-cost, method of cerebrovascular perfusion that reduces the number of animals required to generate robust data from latex-casted brain tissue.



中文翻译:

一种小鼠大脑离体乳胶血管造影的新方法

介绍

乳胶灌注是研究动物大脑中脑血管病理学的有效工具。它提供关于离体分析的低成本、高保真解剖信息,并可用于研究多种状态。然而,目前的乳胶浇注和组织清除方法不允许在样品处理后进行免疫组织化学分析。这导致需要增加动物数量才能获得足够数据的实验。

新方法

在本文中,我们提出了一种改进的乳胶灌注和组织处理协议,用于脑血管系统的离体分析。该方法包括用液体乳胶注射动脉树,然后用鳞片溶液清除组织。

结果

我们的结果证明了小鼠脑血管树的有效和可靠的灌注,高度详细地渲染了大脑的动脉形态,同时允许对样本进行后灌注、免疫组织化学。

与现有方法的比较

我们的技术允许对铸造脑血管组织进行后灌注、病理分析,从而绕过了先前乳胶血管造影方案的局限性。

结论

该协议提供了一种可靠、低成本的脑血管灌注方法,可减少从乳胶铸造脑组织生成可靠数据所需的动物数量。

更新日期:2021-08-31
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