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Detection of a NDM-5-producing Klebsiella pneumoniae sequence type 340 (CG258) high-risk clone in swine
Veterinary Microbiology ( IF 3.3 ) Pub Date : 2021-08-30 , DOI: 10.1016/j.vetmic.2021.109218
Wenbo Zhao 1 , Shihong Li 1 , Stefan Schwarz 2 , Aijuan Li 1 , Hong Yao 1 , Xiang-Dang Du 1
Affiliation  

The emergence and rapid increase of carbapenem-resistant Enterobacteriaceae among food-producing animals poses a serious threat to public health. The aim of this study was to investigate the presence and dissemination of blaNDM-5 in porcine Klebsiella pneumoniae isolates. Of 19 meropenem-resistant K. pneumoniae isolates, 18 were blaNDM-5-positive and one carried blaNDM-1. Susceptibility testing indicated that all blaNDM-carrying K. pneumoniae showed a multiple drug resistance (MDR) profile. The blaNDM-5 gene was located on a conjugative IncX3 plasmid of ∼46-kb in all 18 blaNDM-5-carrying isolates. MLST analysis revealed that ST340 (n = 8), a member of the worldwide existing high-risk epidemic clonal group 258 (CG258), was predominant. Furthermore, whole genome sequence (WGS) analysis for one representative ST340 K. pneumoniae Kp19110124 showed a MDR profile for a wide range of antimicrobial agents, including meropenem, various cephalosporins, azteonam, gentamicin, ciprofloxacin, and florfenicol. Phylogenetic analysis exhibited that K. pneumoniae ST340 strains were clustered into one branch, which had spread across host species and across continents. Among them, K. pneumoniae Kp19110124 displayed a very close relationship with a clinical isolate collected from a patient in Canada. In conclusion, these results reveal the presence of a blaNDM-5-carrying conjugative IncX3 type plasmid into K. pneumoniae ST340 clone, which then may accelerate the dissemination of the blaNDM-5 gene in porcine K. pneumoniae isolates. The detection of high-risk blaNDM-5-carrying K. pneumoniae ST340 in food-producing animal poses a serious threat to public health.



中文翻译:

在猪中检测出产 NDM-5 的肺炎克雷伯菌序列 340 型 (CG258) 高危克隆

耐碳青霉烯类肠杆菌科细菌在食用动物中的出现和迅速增加对公众健康构成严重威胁。本研究的目的是调查bla NDM-5在猪肺炎克雷伯菌分离物中的存在和传播。在 19株耐美罗培南肺炎克雷伯菌分离株中,18 株为bla NDM-5阳性,1株携带bla NDM-1。药敏试验表明,所有携带bla NDM 的肺炎克雷伯菌都表现出多重耐药性 (MDR) 特征。该BLA NDM-5在所有 18携带bla NDM-5 的分离株中,基因位于~46-kb 的结合 IncX3 质粒上。MLST 分析显示,ST340(n = 8)是世界范围内现有的高危流行克隆群 258(CG258)的成员,占主导地位。此外,对一个具有代表性的 ST340肺炎克雷伯菌Kp19110124 的全基因组序列 (WGS) 分析显示了多种抗菌药物的 MDR 谱,包括美罗培南、各种头孢菌素、阿兹托南、庆大霉素、环丙沙星和氟苯尼考。系统发育分析表明,肺炎克雷伯菌ST340 菌株聚集成一个分支,该分支已跨越宿主物种和跨大陆传播。其中,肺炎克雷伯菌Kp19110124 与从加拿大一名患者身上采集的临床分离株表现出非常密切的关系。总之,这些结果揭示了携带bla NDM-5 的结合 IncX3 型质粒存在于肺炎克雷伯菌ST340 克隆中,这可能会加速bla NDM-5基因在猪肺炎克雷伯菌分离物中的传播。在食用动物中检测到携带高危bla NDM-5 的肺炎克雷伯菌ST340 对公众健康构成严重威胁。

更新日期:2021-09-02
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