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Warfarin Induced Quenching of the Carbon Quantum Dots: Mechanism Study and Warfarin Sensor Construction
Journal of Fluorescence ( IF 2.7 ) Pub Date : 2021-08-30 , DOI: 10.1007/s10895-021-02804-2
Mohamad Mahani 1 , Moazame Kordi 1
Affiliation  

The mechanism of the fluorescence quenching of the CQDs by warfarin was determined and based on this study a simple, low cost and highly sensitive nanosensor was developed for determination of Warfarin in plasma samples. The carbon quantum dots with 3.5 µs lifetime (halflife of 2.4 µs) were synthesized by hydrothermal method and characterized. The fluorescence rate constant of 4.5 × 104 s−1 and quenching rate constant of 6.18 × 104 s−1 (from 10 μM warfarin that result in 17% lifetime reduction) was calculated. High quenching efficiency results in 21.63 L mmol−1 Stern–Volmer constant and the study of pH and temperature also confirm the dynamic quenching mechanism. The second order rate constant of 6.18 × 104 L mmol−1 s−1 was obtained for collisions between CQDs and warfarin. Based on this mechanism, a simple, low cost and very sensitive warfarin nanosensor was developed with calibration sensitivity of 21.63 L mmol−1, working range of 0.10 – 12.00 μM and detection limit of 0.01 μM.



中文翻译:

华法林诱导碳量子点猝灭:机理研究和华法林传感器构建

确定了华法林对 CQD 的荧光猝灭机制,并在此研究的基础上开发了一种简单、低成本和高灵敏度的纳米传感器,用于测定血浆样品中的华法林。采用水热法合成了寿命为 3.5 µs(半衰期为 2.4 µs)的碳量子点并进行了表征。计算了 4.5 × 10 4  s -1的荧光速率常数和 6.18 × 10 4  s -1的猝灭速率常数(来自导致 17% 寿命缩短的 10 μM 华法林)。高猝灭效率导致 21.63 L mmol -1 Stern-Volmer 常数,pH 和温度的研究也证实了动态猝灭机制。二阶速率常数为 6.18 × 10对于 CQD 和华法林之间的碰撞,获得了4 L mmol -1  s -1 。基于该机理,研制了一种简单、低成本、灵敏度高的华法林纳米传感器,其校准灵敏度为21.63 L mmol -1,工作范围为0.10-12.00 μM,检测限为0.01 μM。

更新日期:2021-08-31
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