ABSTRACT

This work was aimed to study the opportunity of transgenic oilseed rape (Brassica napus L.), expressing the gene of antimicrobial peptide cecropin P1 (cecP1) to be inhabited with associative microorganisms Methylobacterium mesophilicum and Pseudomonas aureofaciens. Previously, resistance was demonstrated to the microbial pathogens:Erwinia carotovora B15, Pseudomonas syringae, Fusarium oxysporum, and Botrytis cinerea. Analysis of the fatty acid composition of transgenic plant seeds showed an increase in the proportion of unsaturated fatty acids. Plants studied showed increased photosynthetic activity in oxidative stress induced by paraquat instead of in oxidative stress and UV radiation compared to control ones. A study of the interaction of transgenic plants with the associative microorganisms showed that bacteria were located in all initially colonized plants, as well as in plants obtained after several passages of microproliferation. Accordingly, transgenic plants, containing the cecP1 gene were actively colonized by associative bacteria, indicating their safety for associative bacteria, indicating their safety for beneficial microorganisms.

摘要

本工作旨在研究转基因油菜（Brassica napus L.）表达抗菌肽天蚕素P1（cecP1）基因与嗜温甲基杆菌和金黄色假单胞菌共生的机会。此前，已证明对以下微生物病原体具有抗性：欧文氏菌B15、丁香假单胞菌、尖孢镰刀菌和灰葡萄孢. 对转基因植物种子脂肪酸组成的分析表明，不饱和脂肪酸的比例有所增加。与对照植物相比，所研究的植物在百草枯诱导的氧化应激中的光合活性增强，而不是在氧化应激和紫外线辐射中。对转基因植物与缔合微生物相互作用的研究表明，细菌存在于所有最初定殖的植物中，以及在经过几次微增殖后获得的植物中。因此，含有cecP1基因的转基因植物被缔合细菌积极定植，表明它们对缔合细菌的安全性，表明它们对有益微生物的安全性。