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Establishment of a resource recycling strategy by optimizing isobutanol production in engineered cyanobacteria using high salinity stress
Biotechnology for Biofuels ( IF 6.3 ) Pub Date : 2021-08-30 , DOI: 10.1186/s13068-021-02023-8
Xiao-Xi Wu 1 , Jian-Wei Li 1 , Su-Fang Xing 1 , Hui-Ting Chen 1 , Chao Song 1 , Shu-Guang Wang 1 , Zhen Yan 1, 2
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Isobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO2 into bio-based fuels have drawn considerable attention due to their lower feedstock cost and more ecofriendly refining process. Although autotrophic cyanobacteria have been genetically modified for isobutanol biosynthesis, there is a lack of stable and convenient strategies to improve their production. In this study, we first engineered Synechococcus elongatus for isobutanol biosynthesis by introducing five exogenous enzymes, reaching a production titer of 0.126 g/L at day 20. It was then discovered that high salinity stress could result in a whopping fivefold increase in isobutanol production, with a maximal in-flask titer of 0.637 g/L at day 20. Metabolomics analysis revealed that high salinity stress substantially altered the metabolic profiles of the engineered S. elongatus. A major reason for the enhanced isobutanol production is the acceleration of lipid degradation under high salinity stress, which increases NADH. The NADH then participates in the engineered isobutanol-producing pathway. In addition, increased membrane permeability also contributed to the isobutanol production titer. A cultivation system was subsequently developed by mixing synthetic wastewater with seawater to grow the engineered cyanobacteria, reaching a similar isobutanol production titer as cultivation in the medium. High salinity stress on engineered cyanobacteria is a practical and feasible biotechnology to optimize isobutanol production. This biotechnology provides a cost-effective approach to biofuel production, and simultaneously recycles chemical nutrients from wastewater and seawater.

中文翻译:

利用高盐胁迫优化工程蓝藻异丁醇生产,建立资源循环利用策略

异丁醇是一种具有许多优点的有吸引力的生物燃料。将二氧化碳转化为生物基燃料的第三代生物炼油厂因其较低的原料成本和更环保的炼油工艺而备受关注。尽管自养蓝藻已被基因改造用于异丁醇生物合成,但缺乏稳定和方便的策略来提高其产量。在这项研究中,我们首先通过引入五种外源酶来设计用于异丁醇生物合成的细长聚球藻,在第 20 天达到 0.126 g/L 的生产效价。然后发现高盐度胁迫可能导致异丁醇产量增加五倍,在第 20 天的最大瓶内滴度为 0.637 g/L。代谢组学分析表明,高盐度胁迫显着改变了工程细长链球菌的代谢特征。异丁醇产量增加的一个主要原因是在高盐度胁迫下脂质降解加速,这会增加 NADH。然后 NADH 参与工程化的异丁醇生产途径。此外,增加的膜渗透性也有助于异丁醇生产滴度。随后开发了一种培养系统,将合成废水与海水混合以培养工程蓝藻,达到与培养基中培养相似的异丁醇生产滴度。工程蓝藻的高盐胁迫是优化异丁醇生产的实用且可行的生物技术。
更新日期:2021-08-30
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