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Protective role of lycopene in experımental allergic rhinitis in rats
International Journal of Pediatric Otorhinolaryngology ( IF 1.5 ) Pub Date : 2021-08-30 , DOI: 10.1016/j.ijporl.2021.110905
Halil Polat 1 , Mustafa Sagıt 2 , Seren Gulsen Gurgen 3 , Mehmet Yasar 2 , Ibrahim Ozcan 2
Affiliation  

Objective

We investigate whether lycopene has a protective effect in an experimental rat model of allergic rhinitis.

Methods

Experimental animals (65 rats) were randomized to 7 groups (Sham-Control, Lycopene 10 mg/kg/day, Lycopene 20 mg/kg/day, Intranasal lycopene drops, Intranasal steroid, Corn oil, Allergic Rhinitis). Rats were sensitized by administering of ovalbumin intraperitoneally and intranasally. In addition to ovalbumin; lycopene, corn oil and steroids were given to the relevant groups. Nasal symptom scores of the rats were recorded throughout the study. At end of the study, after intracardiac blood sample collection, all rats were sacrificed, and nasal tissues were examined histopathologically. Serum total immunoglobulin E (IgE) and ovalbumin (OVA) specific IgE were studied from all rats before and after the study.

Results

There was a statistically significant increase (p < 0.05) in OVA specific IgE values measured before and after the study in all groups except the sham group. In serum total IgE values; there was a statistically significant increase after treatment in allergic rhinitis, corn oil, lycopene 10 mg and intranasal lycopene drops group, but other groups did not show any significant change. Histopathological study with hematoxylin-eosin staining and cyclooxygenase-2 (COX-2), matrix metalloproteinase-9 (MMP-9), vasoactive intestinal peptide (VIP) expression found that lycopene suppresses inflammation with both nasal administration and increased dose. Nasal symptom scores were observed to decrease significantly in all lycopene and steroid groups compared to allergic rihinits and corn groups.

Conclusion

It was determined that lycopene were effective in the treatment of allergic rhinitis, and this effect was found to be stronger with increasing doses of lycopene.



中文翻译:

番茄红素对大鼠实验性变应性鼻炎的保护作用

客观的

我们研究番茄红素是否在过敏性鼻炎实验大鼠模型中具有保护作用。

方法

实验动物(65 只大鼠)被随机分为 7 组(假对照组、番茄红素 10 mg/kg/天、番茄红素 20 mg/kg/天、鼻内番茄红素滴剂、鼻内类固醇、玉米油、过敏性鼻炎)。通过腹膜内和鼻内施用卵清蛋白使大鼠致敏。除了卵清蛋白;相关组给予番茄红素、玉米油和类固醇。在整个研究过程中记录大鼠的鼻部症状评分。研究结束时,采集心内血样后,处死所有大鼠,对鼻组织进行组织病理学检查。在研究前后对所有大鼠的血清总免疫球蛋白 E (IgE) 和卵清蛋白 (OVA) 特异性 IgE 进行了研究。

结果

除假手术组外,所有组在研究前后测量的 OVA 特异性 IgE 值均有统计学意义的增加 (p < 0.05)。血清总 IgE 值;过敏性鼻炎、玉米油、番茄红素10mg和番茄红素滴鼻组治疗后有统计学意义的增加,其他组无明显变化。苏木精-伊红染色和环氧合酶 2 (COX-2)、基质金属蛋白酶 9 (MMP-9)、血管活性肠肽 (VIP) 表达的组织病理学研究发现,番茄红素通过鼻腔给药和增加剂量均能抑制炎症。与过敏性鼻炎和玉米组相比,观察到所有番茄红素和类固醇组的鼻症状评分显着降低。

结论

确定番茄红素对治疗过敏性鼻炎有效,并且发现这种效果随着番茄红素剂量的增加而增强。

更新日期:2021-08-31
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