当前位置: X-MOL 学术Cancer Lett. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
EMMPRIN in extracellular vesicles from peritoneal mesothelial cells stimulates the invasion activity of diffuse-type gastric cancer cells
Cancer Letters ( IF 9.7 ) Pub Date : 2021-08-30 , DOI: 10.1016/j.canlet.2021.08.031
Atsushi Sugimoto 1 , Tomohisa Okuno 1 , Yuichiro Miki 1 , Gen Tsujio 1 , Tomohiro Sera 1 , Yurie Yamamoto 2 , Shuhei Kushiyama 1 , Sadaaki Nishimura 1 , Kenji Kuroda 1 , Shingo Togano 1 , Koji Maruo 1 , Hiroaki Kasashima 1 , Masaichi Ohira 3 , Masakazu Yashiro 1
Affiliation  

Peritoneal metastasis of gastric cancer (GC) results in extremely poor prognoses. The peritoneal cavity is covered by a monolayer of peritoneal mesothelial cells (PMCs). Interactions between GC cells and PMCs might play a pivotal role in peritoneal metastasis. Extracellular vesicles (EVs) correlate with intercellular communication. Although intercellular communication between cancer cells and PMCs might be associated with the peritoneal metastatic process, the role of EVs from PMCs remains unclear. We investigated the effects of EVs from PMCs on GC cells. Three GC cell lines (OCUM-12, NUGC-3, and MKN74) and four mesothelial cell lines were used. The effects of EVs derived from the PMCs on the invasion and migration of GC cells were evaluated by Matrigel invasion assay. Factors contained in the PMC EVs were analyzed; extra-cellular matrix metalloproteinase inducer (EMMPRIN) was detected in the EVs. The effects of an EMMPRIN inhibitor on the invasion-stimulating activity of EVs were examined. The EMMPRIN expressions of 110 GCs were evaluated by immunohistochemistry. PMC EVs significantly promoted the invasion of diffuse-type GC cells, i.e., OCUM-12 and NUGC-3 cells. EMMPRIN in the EVs stimulated the invasion of OCUM-12 and NUGC-3 cells. The invasion-stimulating activity of PMC EVs was inhibited by the EMMPRIN inhibitor. A high EMMPRIN expression in PMCs was significantly associated with worse cancer-specific survival and peritoneal-recurrence-free survival. EMMPRIN in EVs from PMCs might stimulate the malignant progression of diffuse-type GC. EMMPRIN might be a useful prognostic marker of recurrence in GC patients.



中文翻译:

腹膜间皮细胞胞外囊泡中的EMMPRIN刺激弥漫型胃癌细胞的侵袭活性

胃癌 (GC) 的腹膜转移导致预后极差。腹腔由单层腹膜间皮细胞 (PMC) 覆盖。GC 细胞和 PMC 之间的相互作用可能在腹膜转移中起关键作用。细胞外囊泡 (EV) 与细胞间通讯相关。尽管癌细胞和 PMC 之间的细胞间通讯可能与腹膜转移过程有关,但来自 PMC 的 EV 的作用仍不清楚。我们研究了来自 PMC 的 EV 对 GC 细胞的影响。使用了三种 GC 细胞系(OCUM-12、NUGC-3 和 MKN74)和四种间皮细胞系。通过基质胶侵袭试验评估源自 PMC 的 EV 对 GC 细胞侵袭和迁移的影响。分析了 PMC EV 中包含的因素;在 EV 中检测到细胞外基质金属蛋白酶诱导剂(EMMPRIN)。检查了 EMMPRIN 抑制剂对 EV 侵袭刺激活性的影响。通过免疫组织化学评估110个GC的EMMPRIN表达。PMC EVs显着促进了弥漫型GC细胞的侵袭,即OCUM-12和NUGC-3细胞。EV 中的 EMMPRIN 刺激了 OCUM-12 和 NUGC-3 细胞的侵袭。EMMPRIN 抑制剂抑制 PMC EVs 的侵袭刺激活性。PMC 中 EMMPRIN 的高表达与较差的癌症特异性生存率和无腹膜复发生存率显着相关。来自 PMC 的 EV 中的 EMMPRIN 可能会刺激弥漫型 GC 的恶性进展。EMMPRIN 可能是 GC 患者复发的有用预后标志物。

更新日期:2021-09-03
down
wechat
bug