Salt stress is an important abiotic factor that causes severe losses in soybean yield and quality. Therefore, breeding salt-tolerant soybean germplasm resources via genetic engineering has gained importance. Aspergillus glaucus, a halophilic fungus that exhibits significant tolerance to salt, carries the gene AgGlpF. In this study, we used the soybean cotyledonary node transformation method to transfer the AgGlpF gene into the genome of the soybean variety Williams 82 to generate salt-tolerant transgenic soybean varieties. The results of PCR, Southern blot, ddPCR, and RT-PCR indicated that AgGlpF was successfully integrated into the soybean genome and stably expressed. When subjected to salt stress conditions via treatment with 250 mM NaCl for 3 d, the transgenic soybean plants showed significant tolerance compared with wild-type plants, which exhibited withering symptoms and leaf abscission after 9 d. The results of this study indicated that the transfer of AgGlpF into the genome of soybean plants produced transgenic soybean with significantly improved salt stress tolerance.

盐胁迫是导致大豆产量和质量严重损失的重要非生物因素。因此，通过基因工程培育耐盐大豆种质资源具有重要意义。Aspergillus glaucus是一种嗜盐真菌，对盐表现出显着的耐受性，携带基因AgGlpF。本研究采用大豆子叶节转化方法将AgGlpF基因转入大豆品种Williams 82的基因组中，产生耐盐转基因大豆品种。PCR、Southern印迹、ddPCR和RT-PCR的结果表明AgGlpF成功整合到大豆基因组中并稳定表达。经250 mM NaCl处理3 d的盐胁迫条件下，转基因大豆植株与野生型植株相比表现出显着的耐受性，9 d后出现枯萎症状和叶片脱落。这项研究的结果表明，将AgGlpF转移到大豆植物的基因组中产生了具有显着改善盐胁迫耐受性的转基因大豆。