Carcinoembryonic antigen (CEA) is a key serum tumor marker which is overexpressed in all types of adenocarcinomas. Therefore, establish the ultrasensitive, accurate and rapid method for CEA detection is essential for reducing the mortality of cancer. Here, a bimetallic organic framework Cu/UiO-66 was synthesized through the simple two-step hydrothermal method and used to construct a “fluorescence turn-on” analytical method for CEA detection. Cu/UiO-66 can adsorb CEA aptamers modified with FAM (CEA/FAM-Apt) and take place photoinduced electron transfer (PET) between Cu/UiO-66 and FAM, resulting in the fluorescence of the FAM is quenched. When CEA is present, CEA and CEA/FAM-Apt are tightly combined, making CEA/FAM-Apt far away from the Cu/UiO-66 surface. As a result, the fluorescence intensity of the system was significantly restored. Under optimal conditions, the proposed “fluorescence turn-on” method can detect CEA as low as 0.01 ng mL⁻¹ in a range of 0.01–0.3 ng mL⁻¹. Besides, this analytical method owns good selectivity, reproducibility and serum applicability, which provides a new platform for the direct detection of clinical diagnosis-related markers.

癌胚抗原 (CEA) 是一种关键的血清肿瘤标志物，在所有类型的腺癌中均过度表达。因此，建立超灵敏、准确、快速的CEA检测方法对于降低癌症死亡率至关重要。在这里，通过简单的两步水热法合成了双金属有机骨架 Cu/UiO-66，并用于构建用于 CEA 检测的“荧光开启”分析方法。Cu/UiO-66 可以吸附 FAM 修饰的 CEA 适配体（CEA/FAM-Apt），并在 Cu/UiO-66 和 FAM 之间发生光诱导电子转移（PET），导致 FAM 的荧光被淬灭。当CEA存在时，CEA和CEA/FAM-Apt紧密结合，使得CEA/FAM-Apt远离Cu/UiO-66表面。结果，系统的荧光强度显着恢复。^-1在 0.01–0.3 ng mL ^-1的范围内。此外，该分析方法具有良好的选择性、重现性和血清适用性，为临床诊断相关标志物的直接检测提供了新的平台。