当前位置: X-MOL 学术Fungal Genet. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Sequencing of non-virulent strains of Fusarium fujikuroi reveals genes putatively involved in bakanae disease of rice
Fungal Genetics and Biology ( IF 3 ) Pub Date : 2021-08-28 , DOI: 10.1016/j.fgb.2021.103622
Edoardo Piombo 1 , Marco Rosati 1 , Martina Sanna 1 , Monica Mezzalama 1 , Maria Lodovica Gullino 1 , Davide Spadaro 1
Affiliation  

Bakanae, one of the most important diseases of rice, is caused by the fungal pathogen Fusarium fujikuroi. The elongation of internodes is the most common symptom induced by the pathogen, and it is related to the production of gibberellins. Despite this, the pathogenicity mechanism of F. fujikuroi is still not completely clear, and there are some strains inducing stunting instead of elongation. Even if there are relatively many genomes of F. fujikuroi strains available in online databases, none of them belongs to an isolate of proven non-virulence, and therefore there has been no comparative genomics study conducted between virulent and non-virulent strains. In the present work, the genomes of non-virulent strain SG4 and scarcely virulent strain C2S were compared to the ones of 12 available virulent isolates. Genes present in the majority of available virulent strains, but not in the non-virulent one, underwent functional annotation with multiple tools, and their expression level during rice infection was checked using pre-existing data. Nine genes putatively related to pathogenicity in F. fujikuroi were identified throughout comparative and functional analyses. Among these, many are involved in the degradation of plant cell wall, which is poorly studied in F. fujikuroi-rice interactions. Three of them were validated through qPCR, showing higher expression in the virulent strain and low to no expression in the low virulent and non virulent strains during rice infection. This work helps to clarify the mechanisms of pathogenicity of F. fujikuroi on rice.



中文翻译:

Fusarium fujikuroi无毒株的测序揭示了与水稻bakanae病有关的基因

Bakanae 是水稻最重要的病害之一,由真菌病原体Fusarium fujikuroi 引起。节间伸长是病原菌最常见的症状,与赤霉素的产生有关。尽管如此,F. fujikuroi的致病机制仍不完全清楚,有些菌株会导致发育迟缓而不是伸长。即使F. fujikuroi的基因组相对较多在线数据库中可用的菌株,它们都不属于已证明无毒力的分离株,因此没有在毒性和非毒性菌株之间进行比较基因组学研究。在目前的工作中,将无毒株 SG4 和弱毒株 C2S 的基因组与 12 种可用的毒株进行了比较。存在于大多数可用毒株中的基因,但不存在于非毒株中,使用多种工具进行了功能注释,并使用预先存在的数据检查了它们在水稻感染期间的表达水平。九个与F. fujikuroi致病性相关的基因通过比较和功能分析确定。其中,许多与植物细胞壁的降解有关,这在F. fujikuroi与水稻的相互作用中研究很少。其中三个通过qPCR验证,在水稻侵染过程中,在强毒株中表达较高,在低毒株和无毒株中表达低至无表达。这项工作有助于阐明F. fujikuroi对水稻的致病机制。

更新日期:2021-09-06
down
wechat
bug